Cao Chenyang, Niu Qinya, Chen Jia, Xu Xuebin, Sheng Huanjing, Cui Shenghui, Liu Bin, Yang Baowei
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
College of Chemical Technology, Shijiazhuang University, Shijiazhuang 050035, China.
Microorganisms. 2021 Jan 27;9(2):260. doi: 10.3390/microorganisms9020260.
The emergence of extended-spectrum β-lactamase-producing serovar Enteritidis (ESBL-SE) in humans and foods has gained global attention. In particular, CTX-M-type ESBL-SE are increasingly being detected from various sample types. The aim of this study was to comprehensively analyze the epidemiology and characteristics of -carrying ESBL-SE isolates of clinical origin in Shanghai, China. A total of 292 Enteritidis isolates were recovered from the feces and blood of outpatients and inpatients between 2006 and 2014. Overall, there was a high frequency of cefotaxime-resistant isolates (97.3%), which was significantly higher ( < 0.01) than that of isolates resistant to the other tested antibiotics. All Enteritidis isolates exhibited resistance to ≥1 antibiotic, and 98.0% were multidrug resistant. A total of 233 isolates were identified as ESBL-SE, 166 of which were CTX-M type. Six subtypes of CTX-M-encoding genes were detected, among which (91.6%, 152/166) was the most prevalent genotype. There was high genetic similarity among -positive ESBL-SE. The gene in the ESBL-SE donor strains could be easily transferred into recipient strains. This study highlights that CTX-M-55 should be considered an important surveillance target in Shanghai, China. Cephalosporins, especially cefotaxime, must be used with caution in empirical treatment for infections.
产超广谱β-内酰胺酶肠炎沙门氏菌（ESBL-SE）在人和食品中的出现已引起全球关注。特别是，CTX-M型ESBL-SE越来越多地从各种样本类型中被检测到。本研究的目的是全面分析中国上海临床来源的携带ESBL-SE分离株的流行病学和特征。2006年至2014年间，共从门诊和住院患者的粪便和血液中分离出292株肠炎沙门氏菌。总体而言，头孢噻肟耐药分离株的频率较高（97.3%），显著高于对其他测试抗生素耐药的分离株（P<0.01）。所有肠炎沙门氏菌分离株均表现出对≥1种抗生素耐药，98.0%为多重耐药。共鉴定出233株ESBL-SE，其中166株为CTX-M型。检测到六种CTX-M编码基因亚型，其中CTX-M-55（91.6%，152/166）是最常见的基因型。ESBL-SE阳性菌株之间存在高度的遗传相似性。ESBL-SE供体菌株中的CTX-M基因可轻易转移到受体菌株中。本研究强调，CTX-M-55在中国上海应被视为重要的监测目标。在肠炎沙门氏菌感染的经验性治疗中，必须谨慎使用头孢菌素，尤其是头孢噻肟。
