Rizk Neveen Magdy, Allayeh Abdou Kamal
Environmental Virology Lab., Department of Water Pollution Research, National Research Center, Giza, Egypt.
Iran J Public Health. 2020 Jan;49(1):173-180.
In 2013, WHO estimated the annual diarrheal mortality rate among children less than five years in Egypt was 24 deaths per 100.000, 2% was due to rotavirus infection.
Eight water samples were collected monthly from the Nile water stream passing through Giza over 12 months during June 2016 to May 2017. Totally, ninety-six water samples were collected and concentrated for the detection of rotavirus group A (RV-A) using RT-PCR. Multiplex semi-nested RT-PCR was performed to identify the genotypes P and G of RV-A.
The detection rate of RV-A was 18.75% (18/96), whereas the rate of rotavirus genotypes G and P were 61% (11/18) and 50% (9/18), respectively. Rotaviruses G1P[8] and G1P[4] were the most common genotypes identified in our survey. In addition, the seasonal distribution findings demonstrated that the highest detection rate was 37.5% in the winter season, followed by 20.8%, 12.5% and 4% in spring, autumn and summer, respectively.
Multiplex semi-nested RT-PCR is a useful method for rapid detection and genotyping of RV-A in surface water samples.
2013年，世界卫生组织估计埃及5岁以下儿童的年度腹泻死亡率为每10万人中有24人死亡，其中2% 是由轮状病毒感染所致。
在2016年6月至2017年5月的12个月期间，每月从流经吉萨的尼罗河水系采集8份水样。总共采集了96份水样，并进行浓缩，以使用逆转录聚合酶链反应（RT-PCR）检测A组轮状病毒（RV-A）。采用多重半巢式RT-PCR鉴定RV-A的P和G基因型。
RV-A的检出率为18.75%（18/96），而轮状病毒基因型G和P的检出率分别为61%（11/18）和50%（9/18）。轮状病毒G1P[8]和G1P[4]是我们调查中最常见的基因型。此外，季节性分布结果表明，冬季检出率最高，为37.5%，其次是春季、秋季和夏季，分别为20.8%、12.5%和4%。
多重半巢式RT-PCR是一种用于快速检测地表水样本中RV-A及其基因分型的有用方法。
