Dvořáková Heroldová Monika, Moutelíková Romana, Holá Veronika, Dvořáčková Milada, Prodělalová Jana
Institute of Microbiology, Faculty of Medicine, Masaryk University Anna in Brno, Czech Republic, e-mail:
Klin Mikrobiol Infekc Lek. 2019 Dec;25(4):140-144.
Group A rotavirus (RVA) is one of leading causes of gastroenteritis in children under five years of age and is also an important nosocomial pathogen. In Europe, the most prevalent genotypes of RVA are G1P[8], G2P[4], G3P[8], G4P[8], G9P[8] and G12P[8]. Severe dehydration is the most important complication of RVA gastroenteritis. Each year, rotavirus infection is responsible for 3,000 to 5,000 hospitalizations of children in the Czech Republic. The aim of this study was to detect rotaviruses in patients with suspected acute viral gastroenteritis.
A total of 1 566 stool samples were obtained from patients with acute gastroenteritis from March 2016 to December 2018. All samples were tested by the enzyme immunoassay, rapid immunochromatographic test and quantitative reverse transcription PCR assay to detect RVA. All RVA positive samples were G- and P-typed by Sanger sequencing.
RVA was detected in 13.7 % of the samples (214/1566). The incidence of RVA was 58.9 % (126/214) in males and 41.1 % (88/214) in females. The percentages of positivity ranged from 1 % to 33 % in different age groups. The highest proportion of positive patients was in the age group 4-5 years, 32.6 % (30/92). There was a significant difference in the incidence of rotaviruses between different age groups (p = 0.3946). The prevalent RVA genotypes were G1P[8], G9P[8], G3P[8], G2P[4] and G8P[8]. The detection of the G8P[8] genotype was unusual. The obtained results show that despite the possibility of vaccination, the incidence of RVA infection remains high in the Czech Republic.
A组轮状病毒（RVA）是五岁以下儿童肠胃炎的主要病因之一，也是一种重要的医院感染病原体。在欧洲，RVA最常见的基因型是G1P[8]、G2P[4]、G3P[8]、G4P[8]、G9P[8]和G12P[8]。严重脱水是RVA肠胃炎最重要的并发症。每年，在捷克共和国，轮状病毒感染导致3000至5000名儿童住院。本研究的目的是检测疑似急性病毒性肠胃炎患者中的轮状病毒。
2016年3月至2018年12月期间，从急性肠胃炎患者中总共获取了1566份粪便样本。所有样本均通过酶免疫测定、快速免疫层析试验和定量逆转录PCR试验检测RVA。所有RVA阳性样本通过桑格测序进行G型和P型分型。
在13.7%的样本（214/1566）中检测到RVA。RVA的发病率在男性中为58.9%（126/214），在女性中为41.1%（88/214）。不同年龄组的阳性率在1%至33%之间。阳性患者比例最高的是4至5岁年龄组，为32.6%（30/92）。不同年龄组之间轮状病毒的发病率存在显著差异（p = 0.3946）。流行的RVA基因型为G1P[8]、G9P[8]、G3P[8]、G2P[4]和G8P[8]。G8P[8]基因型的检测并不常见。所得结果表明，尽管有接种疫苗的可能性，但在捷克共和国，RVA感染的发病率仍然很高。
