Yodmeeklin Arpaporn, Khamrin Pattara, Kumthip Kattareeya, Malasao Rungnapa, Ukarapol Nuthapong, Ushijima Hiroshi, Maneekarn Niwat
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Center of Excellence in Emerging and Re-emerging Diarrheal Viruses, Chiang Mai University, Chiang Mai, Thailand.
Arch Virol. 2018 Aug;163(8):2165-2178. doi: 10.1007/s00705-018-3848-0. Epub 2018 Apr 25.
Rotavirus A is a well-known etiological cause of acute gastroenteritis in infants and young children worldwide. In this study, we investigated the prevalence and distribution of RVA genotypes circulating in children with acute gastroenteritis in Thailand from 2010 to 2013. A total of 1,032 fecal specimens were collected from children with an age range from neonatal to 15 years of age and tested for RVA by RT-PCR. Of these, 184 (17.8%) were positive for RVA. The highest detection rate of RVA was found in children aged between 12 and 24 months. The G1P[8] genotype was identified as the most dominant genotype (57.6%), followed by G2P[4] (12.5%), G8P[8] (10.4%), G9P[8] (7.1%), G3P[8] (4.9%), G1P[4] (2.2%), G2P[8] (1.7%), and mixed-infections of G1 and G3 in combination with P[8] (0.5%). In addition, the uncommon human rotavirus strains G4P[6] (1.1%), G9P[19] (0.5%), G12P[4] (0.5%), and G12P[6] (0.5%) were also detected in this study. Interestingly, the unusual G8P[8] strains were detected at a relatively high frequency, and phylogenetic analysis revealed that these G8 strains were genetically closely related to bovine and bovine-like human G8 rotavirus strains reported previously from Thailand, Japan, Vietnam, India and Taiwan. These G8P[8] strains displayed the DS-1-like genotype constellation of G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2 (in the order VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5/6, respectively). Overall, the data indicated a high degree of diversity of RVA genotypes, with the emergence of several uncommon RVA strains in children with acute gastroenteritis in Thailand.
A组轮状病毒是全球婴幼儿急性胃肠炎的一种常见病因。在本研究中，我们调查了2010年至2013年泰国急性胃肠炎患儿中流行的A组轮状病毒（RVA）基因型的患病率和分布情况。总共从年龄范围为新生儿至15岁的患儿中收集了1032份粪便标本，并通过逆转录聚合酶链反应（RT-PCR）检测RVA。其中，184份（17.8%）RVA呈阳性。RVA检测率最高的是12至24个月大的儿童。G1P[8]基因型被确定为最主要的基因型（57.6%），其次是G2P[4]（12.5%）、G8P[8]（10.4%）、G9P[8]（7.1%）、G3P[8]（4.9%）、G1P[4]（2.2%）、G2P[8]（1.7%），以及G1和G3与P[8]的混合感染（0.5%）。此外，本研究还检测到了不常见的人轮状病毒株G4P[6]（1.1%）、G9P[19]（0.5%）、G12P[4]（0.5%）和G12P[6]（0.5%）。有趣的是，不寻常的G8P[8]毒株检测频率相对较高，系统发育分析表明，这些G8毒株在基因上与先前在泰国、日本、越南、印度和中国台湾报道的牛和牛样人G8轮状病毒株密切相关。这些G8P[8]毒株显示出DS-1样的基因型组合G8-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2（分别对应VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5/6的顺序）。总体而言，数据表明RVA基因型具有高度多样性，泰国急性胃肠炎患儿中出现了几种不常见的RVA毒株。
