Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
Talanta. 2020 Aug 1;215:120933. doi: 10.1016/j.talanta.2020.120933. Epub 2020 Mar 17.
In this work, hydrophilic molecularly imprinted nanospheres (MINs) were synthesized via surface imprinting technology and subsequently utilized as dispersant sorbent in matrix solid phase dispersion (MSPD) for the extraction of rhodamine B (RhB) as the illogical food additive dye from different foodstuffs, followed by HPLC analysis. By considering hydrophilicity of target analyte, the sol-gel route was chosen for the preparation of MINs in aqueous media at mild conditions. Most importantly, both synthesis and extraction steps were designed and performed in the line of green chemistry and hazardous waste was eliminated to minimize detrimental impact on the operator health and the environment. The reputable experimental design methodology was adopted to assist the condition optimization of MSPD, which comprehends the significance of the factors and their interactions with the least experimental runs. Under optimized MINs-MSPD-HPLC conditions, the detection limit of RhB was down to 0.14 μg kg and excellent linearity over the wide range of 0.5-10000 μg kg was attained. Furthermore, endogenous RhB was found in the tested food samples with relative standard deviations (RSDs) of ≤4.6%, and satisfactory recoveries were from 83.6 to 96.9%. The simple green MINs-MSPD method holds great potential for determination of trace RhB in complicated solid/semi-solid samples, showing rapidity, accuracy and reliability.
在这项工作中，通过表面印迹技术合成了亲水性分子印迹纳米球（MINs），并随后将其用作基质固相分散（MSPD）中的分散剂吸附剂，从不同的食品中提取作为不合理食品添加剂的罗丹明 B（RhB），然后进行 HPLC 分析。考虑到目标分析物的亲水性，选择溶胶-凝胶法在温和条件下在水介质中制备 MINs。最重要的是，设计并执行了合成和提取步骤，符合绿色化学的原则，并消除了危险废物的产生，以最大程度地减少对操作人员健康和环境的不利影响。采用了著名的实验设计方法来辅助 MSPD 的条件优化，该方法理解了因素的重要性及其与最少实验运行次数的相互作用。在优化的 MINs-MSPD-HPLC 条件下，RhB 的检测限低至 0.14μgkg，并且在 0.5-10000μgkg 的宽范围内实现了极好的线性关系。此外，在所测试的食品样品中发现了内源性 RhB，相对标准偏差（RSD）≤4.6%，回收率令人满意，为 83.6%至 96.9%。简单的绿色 MINs-MSPD 方法在测定复杂的固体/半固体样品中的痕量 RhB 方面具有很大的潜力，具有快速、准确和可靠的特点。
