Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China; College of Animal Science and Technology, Northwest A&F University, Shaanxi, China.
Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Mol Cell Proteomics. 2020 Jun;19(6):1035-1046. doi: 10.1074/mcp.RA120.002017. Epub 2020 Apr 20.
Molecular mechanisms underlying sperm motility have not been fully explained, particularly in chickens. The objective was to identify seminal plasma proteins associated with chicken sperm motility by comparing the seminal plasma proteomic profile of roosters with low sperm motility (LSM, = 4) and high sperm motility (HSM, = 4). Using a label-free MS-based method, a total of 522 seminal plasma proteins were identified, including 386 (∼74%) previously reported and 136 novel ones. A total of 70 differentially abundant proteins were defined, including 48 more-abundant, 15 less-abundant, and seven proteins unique to the LSM group (specific proteins). Key secretory proteins like less-abundant adhesion G-protein coupled receptor G2 (ADGRG2) and more-abundant serine peptidase inhibitor Kazal-type 2 (SPINK2) in the LSM suggested that the corresponding secretory tissues played a crucial role in maintaining sperm motility. Majority (80%) of the more-abundant and five specific proteins were annotated to the cytoplasmic domain which might be a result of higher plasma membrane damage and acrosome dysfunction in LSM. Additionally, more-abundant mitochondrial proteins were detected in LSM seminal plasma associated with lower spermatozoa mitochondrial membrane potential (ΔΨm) and ATP concentrations. Further studies showed that the spermatozoa might be suffering from oxidative stress, as the amount of spermatozoa reactive oxygen species (ROS) were largely enhanced, seminal malondialdehyde (MDA) concentrations were increased, and the seminal plasma total antioxidant capacity (T-AOC) were decreased. Our study provides an additional catalogue of chicken seminal plasma proteome and supports the idea that seminal plasma could be as an indicator of spermatozoa physiology. More-abundant of acrosome, mitochondria and sperm cytoskeleton proteins in the seminal plasma could be a marker of sperm dysfunction and loss of motility. The degeneration of spermatozoa caused by the reduced seminal T-AOC and enhanced oxidative stress might be potential determinants of low sperm motility. These results could extend our understanding of sperm motility and sperm physiology regulation.
精子运动的分子机制尚未得到充分解释，特别是在鸡中。本研究旨在通过比较低精子运动（LSM，n = 4）和高精子运动（HSM，n = 4）公鸡的精液蛋白质组图谱，鉴定与鸡精子运动相关的精液蛋白质。使用无标签 MS 方法，共鉴定出 522 种精液蛋白质，包括 386 种（约 74%）先前报道的和 136 种新蛋白质。共定义了 70 种差异丰富的蛋白质，包括 48 种更多丰富、15 种更少丰富和 7 种仅存在于 LSM 组（特异性蛋白质）的蛋白质。LSM 中较少丰富的粘附 G 蛋白偶联受体 G2（ADGRG2）和更多丰富的丝氨酸肽酶抑制剂 Kazal 型 2（SPINK2）等关键分泌蛋白表明，相应的分泌组织在维持精子运动中起着至关重要的作用。大多数（80%）更多丰富和 5 种特异性蛋白质被注释为细胞质域，这可能是由于 LSM 中更高的质膜损伤和顶体功能障碍。此外，在 LSM 精液中还检测到更多丰富的线粒体蛋白质，与较低的精子线粒体膜电位（ΔΨm）和 ATP 浓度有关。进一步的研究表明，精子可能受到氧化应激的影响，因为精子中活性氧（ROS）的数量大大增加，精液丙二醛（MDA）浓度增加，而精液总抗氧化能力（T-AOC）降低。本研究提供了鸡精液蛋白质组的额外目录，并支持精液可作为精子生理的指标的观点。在精液中更多丰富的顶体、线粒体和精子细胞骨架蛋白质可能是精子功能障碍和运动丧失的标志。由于精液 T-AOC 减少和氧化应激增强导致的精子退化可能是低精子运动的潜在决定因素。这些结果可以扩展我们对精子运动和精子生理调节的理解。
