Cedano-Thomas Yenelli, De La Rosa-Vélez Jorge, Bonami Jean Robert, Vargas-Albores Francisco
Facultad de Ciencias Marinas, UABC, Ensenada, Baja California, México.
Pathogens and Environment, UMR 5119, ECOLAG, CNRS/UM2, cc 093, Université Montpellier 2, Montpellier Cedex, France.
Aquac Res. 2010 Sep;41(10):1432-1443. doi: 10.1111/j.1365-2109.2009.02434.x. Epub 2009 Dec 21.
The yellow head virus (YHV) has been reported to be one of most pathogenic viruses for cultivated shrimp; however, serious problems have only been reported in farms in south and southeastern Asian. Recently, a YHV strain was detected in cultivated in Mexican farms that lacked virus-associated mortalities or epizooties, and the animals were apparently healthy. The identity of the virus was confirmed by sequencing replicative and structural protein-encoding regions and comparing with homologous virus sequences. Phylogenic relationships and genetic distances were also determined and, although some differences were observed, an influence on virulence was uncertain. In addition, the expression levels of several transcripts (3CL, POL, GP64 and GP116) were evaluated by quantitative real-time polymerase chain reaction during an experimental infection. Although the transcript showed varying kinetics, viral genes were expressed in infected , demonstrating the replicative capability of this YHV strain.
黄头病毒(YHV)据报道是养殖对虾最具致病性的病毒之一;然而,严重问题仅在南亚和东南亚的养殖场有报道。最近,在墨西哥养殖场养殖的对虾中检测到一种YHV毒株,这些养殖场没有与病毒相关的死亡或疫病流行情况,而且这些对虾显然是健康的。通过对复制和结构蛋白编码区进行测序并与同源病毒序列进行比较,确认了该病毒的身份。还确定了系统发育关系和遗传距离,尽管观察到了一些差异,但对毒力的影响尚不确定。此外,在实验感染期间通过定量实时聚合酶链反应评估了几种转录本(3CL、POL、GP64和GP116)的表达水平。尽管转录本显示出不同的动力学,但病毒基因在受感染的对虾中表达,证明了这种YHV毒株的复制能力。