通过原位杂交方法鉴定长非编码 RNA。
Identification of Long Noncoding RNA by In Situ Hybridization Approaches.
机构信息
IDI-IRCCS, Rome, Italy.
Department of Experimental Medicine, University of Rome "Tor Vergata", Rome, Italy.
出版信息
Methods Mol Biol. 2020;2154:175-185. doi: 10.1007/978-1-0716-0648-3_15.
PMID:32314217
Abstract
In situ hybridization (ISH) and fluorescence in situ hybridization (FISH) techniques enable us to detect the expression of a specific RNA in fixed cells or tissue sections. Here, we describe in detail two procedures adjusted to reveal specifically lncRNAs in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the two different approaches are also shown.
摘要
原位杂交（ISH）和荧光原位杂交（FISH）技术使我们能够检测固定细胞或组织切片中特定 RNA 的表达。在这里，我们详细描述了两种适用于正常人类角质形成细胞和皮肤组织样本中特异性 lncRNA 检测的程序。还展示了两种不同方法获得的结果示例。
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