Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil; National Institute of Basic Optics and Applied to Life Sciences, Physics Institute of São Carlos, University of São Paulo - USP, Av. Trabalhador São-Carlense, 400, Parque Arnold Schimidt, São Carlos, SP, 13566-590, Brazil.
Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil; National Institute of Basic Optics and Applied to Life Sciences, Physics Institute of São Carlos, University of São Paulo - USP, Av. Trabalhador São-Carlense, 400, Parque Arnold Schimidt, São Carlos, SP, 13566-590, Brazil.
Photodiagnosis Photodyn Ther. 2020 Jun;30:101773. doi: 10.1016/j.pdpdt.2020.101773. Epub 2020 Apr 18.
This study aimed to assess the repair of complete surgical tibial fractures fixed with internal rigid fixation (IRF) associated or not to the use of mineral trioxide aggregate (MTA) cement and treated or not with laser (λ = 780 nm, infrared) or LED (λ = 850 ± 10 nm, infrared) lights, 142.8 J/cm per treatment, by means of Raman spectroscopy. Open surgical tibial fractures were created on 18 rabbits (6 groups of 3 animals per group, ∼8 months old) and fractures were fixed with IRF. Three groups were grafted with MTA. The groups of IRF and IRF + MTA that received laser or LED were irradiated every other day during 15 days. Animals were sacrificed after 30 days, being the tibia surgically removed. Raman spectra were collected via the probe at the defect site in five points, resulting in 15 spectra per group (90 spectra in the dataset). Spectra were collected at the same day to avoid changes in laser power and experimental setup. The ANOVA general linear model showed that the laser irradiation of tibial bone fractures fixed with IRF and grafted with MTA had significant influence in the content of phosphate (peak ∼960 cm) and carbonated (peak ∼1,070 cm) hydroxyapatites as well as collagen (peak 1,452 cm). Also, peaks of calcium carbonate (1,088 cm) were found in the groups grafted with MTA. Based on the Raman spectroscopic data collected in this study, MTA has been shown to improve the repair of complete tibial fractures treated with IRF, with an evident increase of collagen matrix synthesis, and development of a scaffold of hydroxyapatite-like calcium carbonate with subsequent deposition of phosphate hydroxyapatite.
本研究旨在评估采用内部刚性固定（IRF）固定的完全外科胫骨骨折的修复情况，这些骨折是否与使用矿化三氧化物聚合体（MTA）水泥以及是否使用激光（λ=780nm，红外线）或 LED（λ=850±10nm，红外线）灯治疗有关，每个治疗的激光能量为 142.8J/cm。通过拉曼光谱法进行研究。在 18 只兔子（每组 3 只动物，约 8 个月大）上进行开放性外科胫骨骨折造模，并用 IRF 固定骨折。其中 3 组用 MTA 移植。IRF 组和 IRF+MTA 组在 15 天内每隔一天接受激光或 LED 照射。30 天后，通过手术切除胫骨处死动物，然后在缺陷部位通过探头收集拉曼光谱，每组获得 15 个光谱（数据集共有 90 个光谱）。为避免激光功率和实验装置发生变化，每天在同一时间收集光谱。一般线性模型的方差分析表明，IRF 固定和 MTA 移植的胫骨骨折激光照射对磷酸盐（峰约 960cm）和碳酸化（峰约 1070cm）羟磷灰石以及胶原（峰 1452cm）的含量有显著影响。此外，在 MTA 移植组中还发现了碳酸钙（1088cm）的峰。基于本研究中收集的拉曼光谱数据，MTA 已被证明可改善 IRF 治疗的完全胫骨骨折的修复，胶原基质合成明显增加，并形成羟磷灰石样碳酸钙支架，随后沉积磷酸盐羟磷灰石。
