Center of Biophotonics, School of Dentistry, Federal University of Bahia, Av. Araújo Pinho, 62, Canela, Salvador, BA, 40110-150, Brazil.
Lasers Med Sci. 2013 May;28(3):815-22. doi: 10.1007/s10103-012-1166-4. Epub 2012 Jul 26.
The aim of the present study was to assess, by Raman spectroscopy and laser fluorescence, the repair of surgical fractures fixed with wire osteosynthesis treated or not with infrared laser (λ780 nm, 50 mW, 4 × 4 J/cm(2) =16 J/cm(2), ϕ=0.5 cm(2), CW) associated or not to the use of hydroxyapatite and guided bone regeneration. Surgical tibial fractures were created under general anesthesia on 15 rabbits that were divided into five groups, maintained on individual cages, at day/night cycle, fed with solid laboratory pelted diet, and had water ad libitum. The fractures in groups II, III, IV, and V were fixed with wires. Animals in groups III and V were grafted with hydroxyapatite (HA) and guided bone regeneration (GBR) technique used. Animals in groups IV and V were irradiated at every other day during 2 weeks (4 × 4 J/cm(2), 16 J/cm(2) =112 J/cm(2)). Observation time was that of 30 days. After animal death, specimens were taken and kept in liquid nitrogen and used for Raman spectroscopy. The Raman results showed basal readings of 1,234.38 ± 220. Groups WO+B+L showed higher readings (1,680.22 ± 822) and group WO+B the lowest (501.425 ± 328). Fluorescence data showed basal readings of 5.83333 ± 0.7. Groups WO showed higher readings (6.91667 ± 0.9) and group WO+B+L the lowest (1.66667 ± 0.5). There were significant differences between groups on both cases (p<0.05). Pearson correlation was negative and significant (R (2) = -0.60; p<0.001), and it was indicative that, when the Raman peaks of calcium hydroxyapatite (CHA) are increased, the level of fluorescence is reduced. It is concluded that the use of near-infrared lasertherapy associated to HA graft and GBR was effective in improving bone healing on fractured bones as a result of the increasing deposition of CHA measured by Raman spectroscopy and decrease of the organic components as shown by the fluorescence readings.
本研究的目的是通过拉曼光谱和激光荧光评估用或不用近红外激光（λ780nm，50mW，4×4J/cm²=16J/cm²，φ=0.5cm²，CW）治疗的 wire 骨外固定术治疗的外科骨折修复情况，该激光联合或不联合使用羟磷灰石和引导骨再生。在全麻下对 15 只兔子进行胫骨外科骨折手术，将它们分为五组，置于单独的笼中，昼夜循环，喂食固体实验室颗粒饮食，自由饮水。第 II、III、IV 和 V 组的骨折用 wire 固定。第 III 和 V 组的动物用羟磷灰石（HA）和引导骨再生（GBR）技术进行移植。第 IV 和 V 组的动物每隔一天接受 2 周的照射（4×4J/cm²，16J/cm²=112J/cm²）。观察时间为 30 天。动物死后，取标本置于液氮中，用于拉曼光谱。拉曼结果显示基础读数为 1234.38±220。WO+B+L 组显示出更高的读数（1680.22±822），而 WO+B 组读数最低（501.425±328）。荧光数据显示基础读数为 5.83333±0.7。WO 组显示出更高的读数（6.91667±0.9），而 WO+B+L 组读数最低（1.66667±0.5）。两种情况下，各组之间均存在显著差异（p<0.05）。Pearson 相关性为负且显著（R²=-0.60；p<0.001），表明当钙羟磷灰石（CHA）的拉曼峰增加时，荧光强度降低。研究结果表明，近红外激光治疗联合 HA 移植和 GBR 可有效改善骨折骨的愈合，这是通过拉曼光谱测量到的 CHA 沉积增加以及荧光读数显示的有机成分减少而得出的。
