Lin Xinda, Gao Han, Xu Yili, Zhang Yuwei, Li Yan, Lavine Mark D, Lavine Laura Corley
Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
Key Laboratory of Marine Food Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.
iScience. 2020 Apr 24;23(4):101040. doi: 10.1016/j.isci.2020.101040. Epub 2020 Apr 10.
Wing polyphenism is a phenomenon in which one genotype can produce two or more distinct wing phenotypes adapted to the particular environment. What remains unknown is how wing pad development is controlled downstream of endocrine signals such as insulin and JNK pathways. We show that genes important in cellular proliferation, cytokinesis, and cell cycle progression are necessary for growth and development of long wings. Wing pad cellular development of the long-winged morph was characterized by a highly structured epithelial layer with microvilli-like structures. Cells of adult short wing pads are largely in the G2/M phase of the cell cycle, whereas those of long wings are largely in G1. Our study is the first to report the comparative developmental and cellular morphology and structure of the wing morphs and to undertake a comprehensive evaluation of the cell cycle genes necessary for wing development of this unique, adaptive life history strategy.
翅多型现象是指一种基因型能够产生两种或更多种适应特定环境的不同翅表型的现象。目前尚不清楚的是，在胰岛素和JNK信号通路等内分泌信号下游，翅芽发育是如何受到调控的。我们发现，在细胞增殖、胞质分裂和细胞周期进程中起重要作用的基因对于长翅的生长和发育是必需的。长翅形态的翅芽细胞发育特征是具有高度结构化的上皮层，带有微绒毛样结构。成年短翅芽的细胞大多处于细胞周期的G2/M期，而长翅的细胞大多处于G1期。我们的研究首次报道了翅形态的比较发育以及细胞形态和结构，并对这种独特的适应性生活史策略的翅发育所必需的细胞周期基因进行了全面评估。
