College of Life Sciences, China Jiliang University, Hangzhou, China, 310018.
STAR Protoc. 2020 Aug 2;1(2):100080. doi: 10.1016/j.xpro.2020.100080. eCollection 2020 Sep 18.
Deciphering cell cycle phases of polyphenic tissues is an important challenge in understanding the cellular mechanism of polymorphism. We use flow cytometry to analyze cell cycle phases of short wings and long wings of the brown planthopper. This provides information on the arresting cell cycle phases in different wing forms. The protocol could be applied to analysis of the cell cycle phases of other polyphenic insects and in different polyphenic tissues after modification. For complete details on the use and execution of this protocol, please refer to Lin et al. (2020).
解析多态组织的细胞周期阶段是理解多态性细胞机制的重要挑战。我们使用流式细胞术分析褐飞虱短翅和长翅的细胞周期阶段。这为不同翅型中细胞周期停滞的阶段提供了信息。该方案可以通过修改后应用于其他多态昆虫和不同多态组织的细胞周期阶段分析。有关该方案使用和执行的完整详细信息，请参阅 Lin 等人（2020 年）。
