Yan Ning, Gai Xiaolei, Xue Lin, Du Yongmei, Shi John, Liu Yanhua
Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China.
Yunnan Tobacco Leaf Company, Kunming 650000, China.
Plants (Basel). 2020 Apr 17;9(4):518. doi: 10.3390/plants9040518.
solanesyl diphosphate synthase 1 (NtSPS1) is the key enzyme in solanesol biosynthesis. However, changes in the solanesol content, plant growth, photosynthesis, and metabolome of tobacco plants after overexpression (OE) have not been previously reported. In the present study, these parameters, as well as photosynthetic gas exchange, chlorophyll content, and chlorophyll fluorescence parameters, were compared between OE and wild type (WT) lines of tobacco. As expected, OE significantly increased solanesol content in tobacco leaves. Although OE significantly increased leaf growth, photosynthesis, and chlorophyll content, the chlorophyll fluorescence parameters in the leaves of the OE lines were only slightly higher than those in the WT leaves. Furthermore, OE resulted in 64 differential metabolites, including 30 up-regulated and 34 down-regulated metabolites, between the OE and WT leaves. Pathway enrichment analysis of these differential metabolites identified differentially enriched pathways between the OE and WT leaves, e.g., carbon fixation in photosynthetic organisms. The maximum carboxylation rate of RuBisCO and the maximum rate of RuBP regeneration were also elevated in the OE line. To our knowledge, this is the first study to confirm the role of in solanesol biosynthesis and its possible functional mechanisms in tobacco.
茄尼醇二磷酸合酶1（NtSPS1）是茄尼醇生物合成中的关键酶。然而，此前尚未报道过烟草植株过表达（OE）后茄尼醇含量、植株生长、光合作用和代谢组的变化。在本研究中，对烟草OE系和野生型（WT）系的这些参数以及光合气体交换、叶绿素含量和叶绿素荧光参数进行了比较。正如预期的那样，OE显著提高了烟草叶片中的茄尼醇含量。虽然OE显著增加了叶片生长、光合作用和叶绿素含量，但OE系叶片的叶绿素荧光参数仅略高于WT叶片。此外，OE导致OE和WT叶片之间有64种差异代谢物，包括30种上调代谢物和34种下调代谢物。对这些差异代谢物的通路富集分析确定了OE和WT叶片之间差异富集的通路，例如光合生物中的碳固定。OE系中核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBisCO）的最大羧化速率和核酮糖-1,5-二磷酸（RuBP）再生的最大速率也有所提高。据我们所知，这是第一项证实其在茄尼醇生物合成中的作用及其在烟草中可能的功能机制的研究。
