Nakano I, Miyazaki K, Funakoshi A, Tateishi K, Hamaoka T, Yajima H
Third Department of Internal Medicine, Kyushu University, Fukuoka, Japan.
Regul Pept. 1988 Nov;23(2):153-9. doi: 10.1016/0167-0115(88)90023-7.
We examined the effect of porcine gastrin-releasing peptide (GRP-27) and other analogous neuropeptides on cholecystokinin (CCK) secretion from the isolated perfused rat duodenum. GRP-27 stimulated CCK secretion in a monophasic pattern and in a dose-dependent manner ranging from 10(-9) M to 10(-6) M, and 10(-7) M of GRP-27 led to an increment of 442 +/- 120.8 fmol/3 min. The stimulatory effect of GRP-27 on CCK was not inhibited by 10(-5) M of atropine. 10(-7) M of neuromedin C and B, analogs of GRP, stimulated CCK secretion to increments of 382 +/- 64.1 and 289 +/- 47.2 fmol/3 min, respectively. Carbachol (10(-9) to 10(-6) M), VIP (10(-9)M), secretin (10(-9)M) and glucose (11 mM) did not stimulate CCK secretion, and the addition of atropine (10(-5)M) to them led to no significant changes. These results suggest that GRP may directly stimulate CCK secretion from the duodenum and work as a non-cholinergic, peptidergic neurotransmitter.
我们研究了猪胃泌素释放肽（GRP-27）及其他类似神经肽对离体灌注大鼠十二指肠胆囊收缩素（CCK）分泌的影响。GRP-27以单相模式并在10⁻⁹M至10⁻⁶M的剂量范围内以剂量依赖性方式刺激CCK分泌，10⁻⁷M的GRP-27导致CCK分泌增加442±120.8 fmol/3分钟。10⁻⁵M的阿托品未抑制GRP-27对CCK的刺激作用。10⁻⁷M的神经介素C和B（GRP的类似物）分别刺激CCK分泌增加至382±64.1和289±47.2 fmol/3分钟。卡巴胆碱（10⁻⁹至10⁻⁶M）、血管活性肠肽（10⁻⁹M）、促胰液素（10⁻⁹M）和葡萄糖（11 mM）均未刺激CCK分泌，并且向它们中添加阿托品（10⁻⁵M）也未导致显著变化。这些结果表明，GRP可能直接刺激十二指肠的CCK分泌，并作为一种非胆碱能肽能神经递质发挥作用。
