Gao Huanhuan, Tang Lijuan, Lin Jianxian, Zhang Wenxin, Li Yongping, Zhang Ping
Department of Ocular Pathology, State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou 510060, Guangdong Province, China.
J Ophthalmol. 2020 Mar 30;2020:7354275. doi: 10.1155/2020/7354275. eCollection 2020.
In this study, we aimed to investigate the presence of Epstein-Barr virus (EBV) in the eyelid sebaceous gland carcinoma (SGC) and its association with the clinicopathologic features.
One hundred and thirty paraffin-embedded SGC specimens were retrieved from the Clinical Pathology Department of Zhongshan Ophthalmic Center. Epstein-Barr virus-encoded RNA (EBER) was detected with in situ hybridization (ISH) using the Leica BOND system autostainer. The age and gender distributions of all patients were analyzed and compared with earlier reports. Pearson's and Fisher's exact tests were used to determine the association between clinicopathological features such as age, gender, laterality eye, tumor basal dimension, degree of tumor differentiation, and EBER positivity. Likewise, the relationship between the grade and tumor basal dimension in EBER-positive SGC of the eyelid was analyzed.
Thirty-four out of one hundred and thirty (26.2%) eyelid SGC specimens were positively stained for EBER. The age range of highest incidence was 46-75 years, and the female to male ratio was 1 : 0.9. No significant correlation was found between EBER-positivity and age ( = 0.5370), gender ( = 0.4758), and degree of tumor differentiation ( = 0.7787). However, EBV positivity was strongly correlated with the right eye ( = 0.0287), the tumor basal dimension ( = 0.0001). EBV positivity grade presented statistically associated with tumor size ( = 0.0329).
We conclude that ISH is a sensitive method to identify EBV in SGC of the eyelid. A possible causal association of EBV in SGC patients is suggested by high frequency of EBER-ISH positivity and its association with the clinicopathologic features.
在本研究中，我们旨在调查眼睑皮脂腺癌（SGC）中EB病毒（EBV）的存在情况及其与临床病理特征的关联。
从中山眼科中心临床病理科获取130例石蜡包埋的SGC标本。使用徕卡BOND系统自动染色仪通过原位杂交（ISH）检测EB病毒编码RNA（EBER）。分析所有患者的年龄和性别分布，并与早期报告进行比较。采用Pearson检验和Fisher精确检验来确定年龄、性别、患侧眼、肿瘤基底尺寸、肿瘤分化程度等临床病理特征与EBER阳性之间的关联。同样，分析眼睑EBER阳性SGC中分级与肿瘤基底尺寸之间的关系。
130例眼睑SGC标本中有34例（26.2%）EBER染色呈阳性。最高发病年龄范围为46 - 75岁，男女比例为1∶0.9。未发现EBER阳性与年龄（P = 0.5370）、性别（P = 0.4758）和肿瘤分化程度（P = 0.7787）之间存在显著相关性。然而，EBV阳性与右眼（P = 0.0287）、肿瘤基底尺寸（P = 0.0001）密切相关。EBV阳性分级与肿瘤大小在统计学上具有相关性（P = 0.0329）。
我们得出结论，ISH是识别眼睑SGC中EBV的一种敏感方法。EBER - ISH阳性的高频率及其与临床病理特征的关联提示SGC患者中EBV可能存在因果关系。
