Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Biological Research Center, Faculty of Science, Imam Hossein University, Tehran, Iran.
Microb Pathog. 2020 Aug;145:104199. doi: 10.1016/j.micpath.2020.104199. Epub 2020 Apr 19.
Enterohemorrhagic Escherichia coli (E. coli O157: H7) is an enteric pathogen, transmitted through contaminated water and food. Pathogenic factors include bacterial adhesion, invasion of intestinal epithelial and epithelium cells. The pathogenicity of EHEC is due to the production of Shiga-like toxin (Stx). This toxin binds to the ribosome and inhibits the synthesis of proteins. EHEC causes hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). The EHEC treatment with antibiotics leads to resistance. The best way to solve this problem is to use specific antibodies and prophylaxis. Egg yolk antibody (IgY) is a suitable method for prophylaxis. Hence, the aim of this study was to investigate the production of IgY against Stx toxin and its prophylaxis.
The produced antibodies were confirmed by SDS-PAGE and ELISA. IgY was obtained at a concentration of about 5 mg/ml (30 mg of each egg) and a purity of more than 90%. Toxin and antibody challenge was performed in mice. The obtained IgY was able to neutralize the effect of Stx at 2 mg/mice.
This challenge showed that an antibody produced with an acceptable percentage was able to neutralize the effect of Stx.
肠出血性大肠杆菌（E. coli O157：H7）是一种肠道病原体，通过污染的水和食物传播。致病因素包括细菌黏附、侵袭肠道上皮细胞和上皮细胞。EHEC 的致病性是由于产生志贺样毒素（Stx）。这种毒素与核糖体结合并抑制蛋白质的合成。EHEC 引起出血性结肠炎（HC）和溶血性尿毒症综合征（HUS）。EHEC 对抗生素的治疗产生耐药性。解决这个问题的最好方法是使用特异性抗体和预防措施。卵黄抗体（IgY）是一种合适的预防方法。因此，本研究的目的是研究针对 Stx 毒素的 IgY 的产生及其预防作用。
通过 SDS-PAGE 和 ELISA 确认了产生的抗体。IgY 的浓度约为 5mg/ml（每个鸡蛋 30mg），纯度超过 90%。在小鼠中进行了毒素和抗体的挑战。获得的 IgY 能够中和 2mg/mice 剂量的 Stx 的作用。
这项挑战表明，用可接受的百分比产生的抗体能够中和 Stx 的作用。
