Mazurov A V, Sinitsyn V E, Repin V S
USSR Cardiology Research Center, Academy of Medical Sciences, Moscow.
Thromb Res. 1988 Dec 15;52(6):573-85. doi: 10.1016/0049-3848(88)90130-2.
The effects of two standard, unfractionated heparin preparations on collagen-induced adhesion and aggregation of platelets were studied by Born aggregometry and scanning electron microscopy (SEM). Heparin from porcine intestinal mucosa (HI) and from bovine lung (HL) added to human PRP in the concentration of 2.5 to 5.0 U/ml (1) did not induce platelet aggregation in suspension by itself but stimulated it in combination with the subthreshold doses of fibrillar human collagen type III (CIII); (2) increased by 1.5-2.0 fold the adhesion, but did not affect platelet spreading on a surface coated with human collagen type IV (CIV); and (3) enlarged by 2.5-3.0 fold the area of the CIII-coated surface covered with aggregates, increasing both the number and the size of surface-bound aggregates. Aspirin blocked platelet aggregation in suspension induced by low, near threshold doses, of fibrillar CIII and by subthreshold doses of CIII in combination with heparin, but had no effect on platelet aggregation induced by high (greater than 10 threshold) doses of CIII. Aspirin failed to decrease platelet adhesion to and spreading on CIV substrate, and formation of surface-bound aggregates on CIII substrate in the absence as well as in the presence of heparin.
采用博恩血小板聚集测定法和扫描电子显微镜（SEM）研究了两种标准的未分级肝素制剂对胶原蛋白诱导的血小板黏附和聚集的影响。将来自猪肠黏膜的肝素（HI）和来自牛肺的肝素（HL）以2.5至5.0 U/ml的浓度添加到人富血小板血浆（PRP）中：（1）其本身不会在悬浮液中诱导血小板聚集，但与低于阈值剂量的III型人纤维状胶原蛋白（CIII）联合使用时会刺激血小板聚集；（2）使黏附增加1.5至2.0倍，但不影响血小板在包被有IV型人胶原蛋白（CIV）的表面上的铺展；（3）使包被有CIII的表面上被聚集体覆盖的面积增大2.5至3.0倍，增加了表面结合聚集体的数量和大小。阿司匹林可阻断低剂量、接近阈值剂量的纤维状CIII以及低于阈值剂量的CIII与肝素联合使用时在悬浮液中诱导的血小板聚集，但对高剂量（大于10倍阈值）的CIII诱导的血小板聚集没有影响。在不存在和存在肝素的情况下，阿司匹林均无法降低血小板对CIV底物的黏附及铺展，以及在CIII底物上表面结合聚集体的形成。
