Lösche W, Mazurov A V, Heptinstall S, Groenewegen W A, Repin V S, Till U
Institute of Pathological Biochemistry, Medical Academy of Erfurt, GDR.
Thromb Res. 1987 Dec 1;48(5):511-8. doi: 10.1016/0049-3848(87)90383-5.
The interaction of platelets with surfaces coated with collagens of type III (C III) or IV (C IV) has been studied by measuring the deposition of 51-Cr-labeled platelets and by scanning electron microscopy (SEM). Experiments were performed using platelet-rich plasma (PRP) and suspensions of gel-filtered platelets (GFP). Platelets were deposited on C III mainly as surface-bound aggregates. In contrast they were deposited on C IV mainly as spread forms of individual cells. Formation of aggregates on C III was more extensive for PRP than for GFP; in contrast platelet spreading on C IV was more extensive for GFP than for PRP. The effects of an extract of the plant feverfew on platelet-collagen interactions were determined. Feverfew extract inhibited the deposition of 51-Cr-labeled platelets on both C III and C IV in a dose-dependent way. Similar concentrations of extract were needed to inhibit the formation of surface-bound aggregates and to inhibit platelet spreading in both PRP and GFP.
通过测量51-铬标记血小板的沉积以及扫描电子显微镜（SEM），研究了血小板与涂有III型（C III）或IV型（C IV）胶原蛋白的表面之间的相互作用。实验使用富含血小板的血浆（PRP）和凝胶过滤血小板（GFP）悬浮液进行。血小板主要以表面结合聚集体的形式沉积在C III上。相比之下，它们主要以单个细胞的铺展形式沉积在C IV上。PRP在C III上形成的聚集体比GFP更广泛；相反，GFP在C IV上的血小板铺展比PRP更广泛。测定了小白菊植物提取物对血小板-胶原蛋白相互作用的影响。小白菊提取物以剂量依赖的方式抑制51-铬标记血小板在C III和C IV上的沉积。在PRP和GFP中，抑制表面结合聚集体的形成和抑制血小板铺展需要相似浓度的提取物。
