Institute for Glycomics, Griffith University, Southport, 4222, Queensland, Australia.
Charles Perkins Centre and The University of Sydney, Sydney, Australia.
Sci Rep. 2020 Apr 22;10(1):6829. doi: 10.1038/s41598-020-63569-5.
Biofilms of the gastroenteric pathogen C. jejuni may serve an important role in the transmission of infection from reservoirs of infection to humans. Herein, we undertook a combinatorial approach examining differential gene expression and protein abundance during biofilm formation in C. jejuni. Biofilms induced a substantial rearrangement of the C. jejuni transcriptome and proteome, with ~600 genes differentially expressed when compared to planktonic cells. Genes and proteins induced in biofilms were involved in iron metabolism and acquisition, cell division, glycan production and attachment, while those repressed were associated with metabolism, amino acid usage, and large tracts of the chemotaxis pathway. We further examined the role of chemotaxis in C. jejuni biofilm formation by examining isogenic strains with deletions of the cheV and cheW signal transduction genes. Both ∆cheV and ∆cheW exhibited a significant decrease in directed motility when compared to wild-type C. jejuni as well as demonstrating an increase in autoagglutination ability and biofilm formation. A subtle difference was also observed between the phenotypes of ∆cheV and ∆cheW mutants, both in motility and biofilm formation. This suggests roles for CheV and CheW and may present signal transduction as a potential method for modulating C. jejuni biofilm formation.
胃肠道病原体 C. jejuni 的生物膜可能在感染源向人类传播感染方面发挥重要作用。在此，我们采用组合方法研究了 C. jejuni 生物膜形成过程中的差异基因表达和蛋白质丰度。生物膜诱导了 C. jejuni 转录组和蛋白质组的大量重排，与浮游细胞相比，有约 600 个基因差异表达。生物膜中诱导的基因和蛋白质参与铁代谢和获取、细胞分裂、聚糖产生和附着，而被抑制的基因与代谢、氨基酸利用以及趋化途径的大部分有关。我们通过检查 CheV 和 CheW 信号转导基因缺失的同基因株系，进一步研究了趋化作用在 C. jejuni 生物膜形成中的作用。与野生型 C. jejuni 相比，ΔcheV 和 ΔcheW 的定向运动能力显著降低，同时自聚集能力和生物膜形成能力增加。在运动和生物膜形成方面，还观察到ΔcheV 和ΔcheW 突变体之间的表型存在细微差异。这表明 CheV 和 CheW 发挥作用，并且信号转导可能是调节 C. jejuni 生物膜形成的潜在方法。
