Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD, Australia.
Virulence. 2010 Sep-Oct;1(5):414-7. doi: 10.4161/viru.1.5.12735.
The human bacterial pathogen Campylobacter jejuni is able to respond to environmental stimuli utilising chemotactic motility. The bacterial senses external molecules via transmembrane sensory proteins called Transducer Like Proteins, TLPs. The specificity of the Tlp1 chemoreceptor (Cj1506c) of C. jejuni as the aspartate receptor, CcaA, and its role in chemotaxis signalling pathway were characterised by genetic and biochemical approaches including amino acid and small molecule arrays, Saturation Transfer Difference NMR spectroscopy, and mutational analysis. Yeast two-hybrid and three-hybrid analysis of protein-protein interactions showed that CcaA chemotactic signal was preferentially passed through CheV, rather than the CheW homologue of the chemotaxis signalling pathway allowing a new model for the C. jejuni chemotactic signalling pathway to be postulated.
人肠道致病菌空肠弯曲菌能够利用趋化运动对环境刺激做出反应。细菌通过跨膜感觉蛋白（称为 Transducer Like Proteins，TLPs）感知外部分子。通过遗传和生化方法（包括氨基酸和小分子阵列、饱和转移差 NMR 光谱学和突变分析）对空肠弯曲菌 Tlp1 趋化感受器（Cj1506c）作为天冬氨酸受体 CcaA 的特异性及其在趋化信号通路中的作用进行了表征。酵母双杂交和三杂交蛋白-蛋白相互作用分析表明，CcaA 趋化信号优先通过 CheV 传递，而不是趋化信号通路的 CheW 同源物，从而提出了空肠弯曲菌趋化信号通路的新模型。
