Plant Polymer Research Unit, USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 N, University Street, Peoria, IL, 61604, USA.
Crop Bioprotection Research Unit, USDA, Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 N, University Street, Peoria, IL, 61604, USA.
Sci Rep. 2020 Apr 22;10(1):6806. doi: 10.1038/s41598-020-63746-6.
Four crop plants known to be hosts for the lepidopteran Trichoplusia ni (soybean, green bean, cotton, and cabbage) were treated with the biopesticide AfMNPV baculovirus in a dosage response assay. Treated soybean had, on average, a 6-fold increase in virus activity compared with the other crops. Leaf trichomes on soybeans were not found to be responsible for the observed increase of insecticidal activity. Three flavonoid compounds (daidzein, genistein, and kaempferol) were uniquely found only in the soybean crop, and were not detected in cotton, cabbage, or green bean plant matter. The individual flavonoid compounds did not cause T ni. mortality in no-virus assays when incorporated into artificial insect diet. The combination of the three flavonoid compounds at leaf level concentrations significantly increased baculovirus activity in diet incorporation assays. When the daidzein, genistein, and kaempferol were added to artificial diet, at 3.5-6.5 × leaf level concentrations, virus activity increased 1.5, 2.3, and 4.2-fold for each respective flavonoid. The soybean flavonoid compounds were found to synergistically improve baculovirus activity against T. ni.
四种已知的作为鳞翅目昆虫斜纹夜蛾（大豆、绿豆、棉花和甘蓝）宿主的作物植物在剂量反应测定中用生物农药 AfMNPV 杆状病毒处理。与其他作物相比，处理过的大豆的病毒活性平均增加了 6 倍。大豆叶片上的毛状体并未发现是导致观察到的杀虫活性增加的原因。三种类黄酮化合物（大豆苷、染料木黄酮和山柰酚）仅在大豆作物中发现，而在棉花、甘蓝或绿豆植物物质中未检测到。当将这些类黄酮化合物分别掺入人工昆虫饮食中时，它们不会导致斜纹夜蛾死亡。当这三种类黄酮化合物以叶片水平浓度组合添加到饮食中时，杆状病毒在饮食掺入测定中的活性显著增加。当将大豆苷、染料木黄酮和山柰酚添加到人工饮食中时，在 3.5-6.5×叶片水平浓度下，每种类黄酮的病毒活性分别增加了 1.5、2.3 和 4.2 倍。发现大豆类黄酮化合物可协同提高杆状病毒对斜纹夜蛾的活性。
