Mehrvar Ali, Ghanbari Solmaz, Söylemezoğlu Gökhan, Toprak Umut
Molecular Entomology (MOLEN) Laboratory, Department of Plant Protection, Faculty of Agriculture, Ankara University, Ankara, Türkiye.
Department of Plant Protection, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.
J Econ Entomol. 2025 Feb 11;118(1):83-92. doi: 10.1093/jee/toae282.
Spodoptera littoralis Boisduval (Lepidoptera: Noctuidae) and Spodoptera exigua Hübner (Lepidoptera: Noctuidae) pose substantial threats to many crops, necessitating the exploration of biopesticides as potential chemical alternatives. One alternative is baculoviruses; however, their instability in the field has hindered their widespread use. Host plant phylloplane affects baculovirus activity at varying levels in different host plants. Formulation contributes significantly to optimizing the baculoviral stability on different phylloplanes against environmental conditions; however, it is expensive and difficult to make in developing or nondeveloped countries. In the current study, we developed a simple tank-mix application (MBF-Tm5) for immediate use, resembling the characteristics of a suspension concentrate formulation for Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) and Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV). We examined their biological activity against 2nd instar larvae first on an artificial diet under laboratory conditions and on eggplant and pepper phylloplane in greenhouse conditions compared to plain viruses. This formulation exhibited no significant improvement in the biological activity of both viruses on an artificial diet under laboratory conditions but significantly improved the biological activity of both viruses on both plants under greenhouse conditions. The original activity remaining (OAR%) of both unformulated and formulated viruses decreased over time under greenhouse conditions; however, the OAR value of both viruses on eggplants was significantly higher than on pepper plants. Overall, the tank-mix simple formulation of baculoviruses might be a great alternative for improved stability in nature, providing better control.
草地贪夜蛾（鳞翅目：夜蛾科）和甜菜夜蛾（鳞翅目：夜蛾科）对许多作物构成了重大威胁，因此有必要探索生物农药作为潜在的化学替代品。一种替代品是杆状病毒；然而，它们在田间的不稳定性阻碍了其广泛应用。寄主植物叶表在不同寄主植物中对杆状病毒活性有不同程度的影响。制剂对优化杆状病毒在不同叶表上抵御环境条件的稳定性有显著作用；然而，在发展中国家或不发达国家，其制作成本高昂且困难。在本研究中，我们开发了一种即使用的简单桶混制剂（MBF-Tm5），其类似于草地贪夜蛾核型多角体病毒（SpliNPV）和甜菜夜蛾多核型多角体病毒（SeMNPV）的悬浮剂制剂特性。我们首先在实验室条件下的人工饲料上，以及在温室条件下的茄子和辣椒叶表上，检测了它们对2龄幼虫的生物活性，并与单纯病毒进行了比较。该制剂在实验室条件下的人工饲料上对两种病毒的生物活性没有显著改善，但在温室条件下对两种植物上的两种病毒的生物活性有显著提高。在温室条件下，未制剂化和制剂化病毒的剩余原始活性（OAR%）均随时间下降；然而，两种病毒在茄子上的OAR值显著高于辣椒植株。总体而言，杆状病毒的桶混简单制剂可能是提高其在自然环境中稳定性的一个很好的替代品，能提供更好的防治效果。
