一种从Tohama菌株中提取外膜囊泡（OMV）的新方法。
A novel method for the extraction of outer membrane vesicles (OMVs) from Tohama strain.
作者信息
Sekhavati Mohammad, Mobarez Ashraf Mohabati, Siadat Seyed Davar, Noofeli Mojtaba
机构信息
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Microbiology Research Center (MRC) Pasteur, Institute of Iran, Tehran, Iran.
出版信息
Iran J Microbiol. 2020 Feb;12(1):37-42.
BACKGROUND AND OBJECTIVES
There are many pertussis outbreaks which is mainly due to the reduction in the immunity of acellular pertussis (aP) vaccines. Therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. Preceding researches have shown that outer membrane vesicles (OMVs) have appropriate specifications, making them a suitable vaccine candidate against pertussis.
MATERIALS AND METHODS
The OMVs were separated by a new serial ultra centrifugation technique. Transmission electron microscopy (TEM) examination, SDS-PAGE, Western blotting and ELISA assay were used to characterize the OMVs.
RESULTS
TEM studies showed the size of the extracted OMVs at 40-200 nm. The presence of pertussis toxin, filamentous hemagglutinin, and pertactin was verified using Western blot and ELISA assay.
CONCLUSION
The presented technique is a simple and effective way to obtain OMVs from So it can be utilized as an appropriate procedure in the development of an OMV-based vaccine against pertussis.
背景与目的
目前有许多百日咳疫情爆发，这主要归因于无细胞百日咳（aP）疫苗免疫力的下降。因此，开发新一代百日咳疫苗至关重要。先前的研究表明，外膜囊泡（OMV）具有合适的特性，使其成为一种合适的抗百日咳疫苗候选物。
材料与方法
通过一种新的连续超速离心技术分离OMV。采用透射电子显微镜（TEM）检查、SDS-PAGE、蛋白质印迹法和酶联免疫吸附测定（ELISA）法对OMV进行表征。
结果
TEM研究显示提取的OMV大小在40 - 200纳米之间。使用蛋白质印迹法和ELISA法验证了百日咳毒素、丝状血凝素和百日咳黏附素的存在。
结论
所提出的技术是从[原文此处缺失相关内容]获得OMV的一种简单有效的方法。因此，它可作为开发基于OMV的抗百日咳疫苗的合适程序加以利用。
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