Department of Chemistry and Biochemistry, USA.
Biomedical Engineering, USA.
Anal Chim Acta. 2019 Aug 13;1065:71-78. doi: 10.1016/j.aca.2019.03.001. Epub 2019 Mar 5.
Whooping cough also called Pertussis is a highly contagious respiratory infection that affects all age populations. Given recent pertussis outbreaks, there is an urgent need for a point-of-care (POC) device for rapid diagnosis of pertussis. Herein, we report a low-cost microfluidic POC device integrated with loop-mediated isothermal amplification (LAMP) technique for the rapid and accurate diagnosis of pertussis. The 3D-printed bioanalyzer housed not only the biochip but also an in-house-developed portable and fully battery-powered heater for rapid POC detection of pertussis, without the need of external electricity. The fluorescence-based results could be rapidly visualized in about one hour by the naked eye without the need for any additional instrumentation. In addition, a simple centrifuge-free sample preparation process was optimized for the efficient lysis of pertussis samples and successfully used for direct detection of bacteria in nasopharyngeal samples. High sensitivity, with a limit of detection (LOD) of 5 DNA copies per LAMP zone, and high specificity were demonstrated. We envision that the microfluidic POC device can be used in various venues such as medical clinics, schools, and other low-resource settings for the fast detection of pertussis.
百日咳，又称百日咳，是一种高度传染性的呼吸道感染，影响所有年龄人群。鉴于最近百日咳的爆发，迫切需要一种即时护理（POC）设备来快速诊断百日咳。在此，我们报告了一种低成本的微流控 POCT 设备，该设备集成了环介导等温扩增（LAMP）技术，用于快速准确地诊断百日咳。3D 打印的生物分析仪不仅容纳了生物芯片，还内置了一个便携式、完全电池供电的加热器，用于快速进行 POCT 检测，无需外部电源。基于荧光的结果可以在大约一个小时内用肉眼快速可视化，无需任何额外的仪器。此外，还优化了一种简单的无离心样品制备过程，以有效裂解百日咳样品，并成功用于直接检测鼻咽样品中的细菌。该方法具有较高的灵敏度，LOD 为每 LAMP 区 5 个 DNA 拷贝，且具有较高的特异性。我们设想，这种微流控 POCT 设备可以在各种场所，如诊所、学校和其他资源匮乏的环境中，用于快速检测百日咳。
