Wang Qin, Zhong Chao, Xiao Han
State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, and Laboratory of Molecular Biochemical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Materials and Physical Biology Division, School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.
Front Bioeng Biotechnol. 2020 Apr 8;8:293. doi: 10.3389/fbioe.2020.00293. eCollection 2020.
Filamentous fungi are considered as unique cell factories for protein production due to the high efficiency of protein secretion and superior capability of post-translational modifications. In this review, we firstly introduce the secretory pathway in filamentous fungi. We next summarize the current state-of-the-art works regarding how various genetic engineering strategies are applied for enhancing protein expression and secretion in filamentous fungi. Finally, in a future perspective, we discuss the great potential of genome engineering for further improving protein expression and secretion in filamentous fungi.
丝状真菌因其高效的蛋白质分泌能力和卓越的翻译后修饰能力，被视为蛋白质生产的独特细胞工厂。在本综述中，我们首先介绍丝状真菌中的分泌途径。接着，我们总结了目前关于如何应用各种基因工程策略来增强丝状真菌中蛋白质表达和分泌的前沿研究成果。最后，从未来展望的角度，我们讨论了基因组工程在进一步提高丝状真菌中蛋白质表达和分泌方面的巨大潜力。
