Rajiv Gandhi Centre for Biotechnology, Jagathy, Trivandrum 695 014, India.
Rajiv Gandhi Centre for Biotechnology, Jagathy, Trivandrum 695 014, India.
Bioresour Technol. 2022 Jan;344(Pt A):126209. doi: 10.1016/j.biortech.2021.126209. Epub 2021 Oct 26.
Filamentous fungi possess versatile capabilities for synthesizing a variety of valuable bio compounds, including enzymes, organic acids and small molecule secondary metabolites. The advancements of genetic and metabolic engineering techniques and the availability of sequenced genomes discovered their potential as expression hosts for recombinant protein production. Remarkably, plant-biomass degrading filamentous fungi show the unique capability to decompose lignocellulose, an extremely recalcitrant biopolymer. The basic biochemical approaches have motivated several industrial processes for lignocellulose biomass valorisation into fermentable sugars and other biochemical for biofuels, biomolecules, and biomaterials. The review gives insight into current trends in engineering filamentous fungi for enzymes, fuels, and chemicals from lignocellulose biomass. This review describes the variety of enzymes and compounds that filamentous fungi produce, engineering of filamentous fungi for biomass valorisation with a special focus on lignocellulolytic enzymes and other bulk chemicals.
丝状真菌具有多种合成有价值生物化合物的能力，包括酶、有机酸和小分子次级代谢物。遗传和代谢工程技术的进步以及测序基因组的可用性揭示了它们作为重组蛋白生产的表达宿主的潜力。值得注意的是，植物生物质降解丝状真菌表现出独特的分解木质纤维素的能力，木质纤维素是一种极其顽固的生物聚合物。基本的生化方法已经推动了几种工业工艺，将木质纤维素生物质转化为可发酵糖和其他生物燃料、生物分子和生物材料。该综述深入探讨了利用木质纤维素生物质工程丝状真菌生产酶、燃料和化学品的当前趋势。本综述描述了丝状真菌产生的各种酶和化合物，以及丝状真菌在生物质增值方面的工程设计，特别关注木质纤维素酶和其他大宗化学品。
