大肠杆菌1型体细胞菌毛突变体的遗传互补分析。
Genetic complementation analysis of Escherichia coli type 1 somatic pilus mutants.
作者信息
Swaney L M, Liu Y P, Ippen-Ihler K, Brinton C C
出版信息
J Bacteriol. 1977 Apr;130(1):506-11. doi: 10.1128/jb.130.1.506-511.1977.
Abstract
A genetic complementation analysis of 75 stable nonpiliated mutants of a type 1 piliated strain of Escherichia coli K-12, AW405, was performed. Strains containing pairs of pil mutations were constructed by the infectious transfer of an F101 plasmid containing one pil mutation into E. coli K-12 AW 405 containing another pil mutation. The presence or absence of type 1 pili on the merodiploid strains was determined by agglutination with type 1 pilus antiserum. All 75 mutants fell into one of four complementation groups. The pattern of complementation defined three cistrons involved in pilus formation, pilA, pilB, and pilC. The fourth complementation group was composed of a large number of mutants defective in both pilA and pilB functions.
摘要
对大肠杆菌K-12的1型菌毛菌株AW405的75个稳定的无菌毛突变体进行了遗传互补分析。通过将含有一个菌毛突变的F101质粒感染性转移到含有另一个菌毛突变的大肠杆菌K-12 AW405中，构建了含有成对菌毛突变的菌株。通过用1型菌毛抗血清进行凝集来确定部分二倍体菌株上是否存在1型菌毛。所有75个突变体都属于四个互补组之一。互补模式确定了参与菌毛形成的三个顺反子，即菌毛A、菌毛B和菌毛C。第四个互补组由大量在菌毛A和菌毛B功能上都有缺陷的突变体组成。
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