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大肠杆菌K-12 I区鞭毛突变体的遗传分析

Genetic analysis of Escherichia coli K-12 region I flagellar mutants.

作者信息

Komeda Y, Silverman M, Simon M

出版信息

J Bacteriol. 1977 Sep;131(3):801-8. doi: 10.1128/jb.131.3.801-808.1977.

DOI:10.1128/jb.131.3.801-808.1977
PMID:330498
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC235534/
Abstract

Flagellar mutants in Escherichia coli region I were obtained by selection for resistance to the flagellotropic phage chi. F' elements carrying this region of the E. coli genome were then constructed. Stable merodiploid strains with a flagellar defect on the exogenate and another on the endogenote were prepared. These merodiploids yielded information on the complementation behavior of mutations in this region. Region I was shown to include at least six cistrons, flaV, flaK, flaL, flaM, flaS, and flaT. Mu-induced and deletion fla mutants were also isolated. By using these mutant strains, the transcriptional order was shown to be flaV-flaK-flaL-flaM-flaS-flaT. The definition of region I fla genes and their transcriptional relationships were confirmed by genetic tests with hybrid A phage carrying fla genes in this region.

摘要

通过筛选对嗜鞭毛噬菌体chi具有抗性，获得了大肠杆菌I区的鞭毛突变体。然后构建了携带大肠杆菌基因组该区域的F'因子。制备了在外源基因上有鞭毛缺陷而在内源基因上也有另一种鞭毛缺陷的稳定部分二倍体菌株。这些部分二倍体提供了该区域突变互补行为的信息。结果表明，I区至少包括六个顺反子，即flaV、flaK、flaL、flaM、flaS和flaT。还分离出了Mu诱导的和缺失的fla突变体。通过使用这些突变菌株，转录顺序显示为flaV-flaK-flaL-flaM-flaS-flaT。携带该区域fla基因的杂交A噬菌体的遗传试验证实了I区fla基因的定义及其转录关系。

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