Department of Physics, Saratov State University, Astrakhanskaya Street 83, 410012 Saratov, Russia.
Laboratory of Biomedical Nanotechnology, I.M. Sechenov First Moscow State Medical University, Bolshaya Pirogovskaya Street 2-4, 119991 Moscow, Russia.
Molecules. 2020 Apr 18;25(8):1875. doi: 10.3390/molecules25081875.
This paper discusses specific features of the interactions of small-diameter liposomes with the cytoplasmic membrane of endothelial cells using in silico methods. The movement pattern of the liposomal drug delivery system was modeled in accordance with the conditions of the near-wall layer of blood flow. Our simulation results show that the liposomes can become stuck in the intercellular gaps and even break down when the gap is reduced. Liposomes stuck in the gaps are capable of withstanding a shell deformation of ~15% with an increase in liposome energy by 26%. Critical deformation of the membrane gives an impetus to drug release from the liposome outward. We found that the liposomes moving in the near-wall layer of blood flow inevitably stick to the membrane. Liposome sticking on the membrane is accompanied by its gradual splicing with the membrane bilayer. This leads to a gradual drug release inside the cell.
本文使用计算机模拟方法探讨了小直径脂质体与内皮细胞质膜相互作用的具体特征。根据血流近壁层的条件，对脂质体药物传递系统的运动模式进行了建模。我们的模拟结果表明，当间隙减小时，脂质体可能会卡在细胞间隙中甚至破裂。卡在间隙中的脂质体能够承受约 15%的壳变形，同时增加 26%的脂质体能量。膜的临界变形促使药物从脂质体向外释放。我们发现，在血流近壁层中运动的脂质体不可避免地会粘在膜上。粘在膜上的脂质体伴随着其与膜双层的逐渐拼接。这导致细胞内药物逐渐释放。
