体外抗利什曼原虫金属中取代卟啉衍生物对前鞭毛体的作用特性。
In Vitro Anti-Leishmanial Effect of Metallic Meso-Substituted Porphyrin Derivatives against Promastigotes Properties.
机构信息
Grupo de Fotoquímica y Fotobiología, Universidad del Atlántico, Puerto Colombia 810007, Colombia.
Grupo de Investigación UNIMOL, Universidad de Cartagena, Cartagena 130001, Colombia.
出版信息
Molecules. 2020 Apr 19;25(8):1887. doi: 10.3390/molecules25081887.
In this study, a family of porphyrins based on 5,10,15,20-Tetrakis(4-ethylphenyl)porphyrin (, Ph) and six metallo-derivatives (Zn(, Ph-Zn), Sn(, Ph-Sn), Mn (, Ph-Mn), Ni (, Ph-Ni), Al (, Ph-Al), and V (, Ph-V)) were tested as photosensitizers for photodynamic therapy against and . The singlet oxygen quantum yield value (Φ) for (-) was measured using 1,3-diphenylisobenzofuran (DPBF) as a singlet oxygen trapping agent and 5,10,15,20-(tetraphenyl)-porphyrin (H2TPP) as a reference standard; besides, parasite viability was estimated by the MTT assay. After metal insertion into the porphyrin core, the Φ increased from 0.76-0.90 and cell viability changed considerably. The Φ and metal type changed the cytotoxic activity. Finally, () showed both the highest Φ (0.90) and the best photodynamic activity against the parasites studied (IC of 1.2 μM).
在这项研究中，以 5,10,15,20-四(4-乙基苯基)卟啉（Ph）为基础，研究了一系列卟啉类化合物及其六种金属衍生物（Zn(Ph-Zn)、Sn(Ph-Sn)、Mn (Ph-Mn)、Ni (Ph-Ni)、Al (Ph-Al)和 V (Ph-V))，用作针对 和 的光动力疗法的光敏剂。使用 1,3-二苯基异苯并呋喃（DPBF）作为单线态氧捕获剂和 5,10,15,20-（四苯基）-卟啉（H2TPP）作为参比标准，测量了 (-) 的单线态氧量子产率（Φ）；此外，通过 MTT 测定法评估寄生虫的活力。金属插入卟啉核后，Φ 从 0.76-0.90 增加，细胞活力发生了显著变化。Φ 和金属类型改变了细胞毒性活性。最后，() 表现出最高的 Φ（0.90）和针对研究寄生虫的最佳光动力活性（IC 为 1.2 μM）。
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