Proverbio Daniela, Perego Roberta, Baggiani Luciana, Ravasio Giuliano, Giambellini Daniela, Spada Eva
Department of Veterinary Medicine (DIMEVET), University of Milan, via dell'Università 6, 26900 Lodi, Italy.
Via Metteotti 9, Almenno San Salvatore, Bergam 24031, Italy.
Animals (Basel). 2020 Apr 20;10(4):716. doi: 10.3390/ani10040716.
Given the endangered status of tigers (), the health of each individual is important and any data on blood chemistry values can provide valuable information alongside the assessment of physical condition. The nature of tigers in the wild makes it is extremely difficult to obtain biological samples from free-living subjects, therefore the values obtained from captive tigers provide very useful data. Serum protein electrophoresis is a useful tool in the diagnosis and monitoring of a number of diseases. In this study, we evaluated agarose gel serum protein electrophoresis on samples from 11 healthy captive tigers. Serum electrophoresis on all 11 tiger samples successfully separated proteins into albumin, α, α, β, β and γ globulin fractions as in other mammals. Electrophoretic patterns were comparable in all tigers. Mean± standard deviation or median and range values obtained for each protein fraction in healthy tigers were, respectively: 3.6 ± 0.2, 0.21 (0.2-0.23), 1.2 ± 0.2, 10.7 ± 0.2, 0.4 (0.3-0.6), 1.2 (1-1.8) gr/dL. The results of this preliminary study provide the first data on serum electrophoretic patterns in tigers and may be a useful diagnostic tool in the health assessment of this endangered species.
鉴于老虎的濒危状态，每只老虎的健康都很重要，任何血液化学值数据都能在评估身体状况的同时提供有价值的信息。野生老虎的习性使得从自由生活的个体获取生物样本极其困难，因此从圈养老虎身上获得的数据非常有用。血清蛋白电泳是诊断和监测多种疾病的有用工具。在本研究中，我们对11只健康圈养老虎的样本进行了琼脂糖凝胶血清蛋白电泳评估。对所有11只老虎样本进行的血清电泳成功地将蛋白质分离为白蛋白、α1、α2、β1、β2和γ球蛋白组分，与其他哺乳动物一样。所有老虎的电泳图谱具有可比性。健康老虎各蛋白质组分的均值±标准差或中位数及范围值分别为：3.6±0.2、0.21（0.2 - 0.23）、1.2±0.2、10.7±0.2、0.4（0.3 - 0.6）、1.2（1 - 1.8）g/dL。这项初步研究的结果提供了老虎血清电泳图谱的首批数据，可能成为评估这种濒危物种健康状况的有用诊断工具。
