Li Zhixing, Lyu Zihao, Ye Qingya, Cheng Jie, Wang Chunyan, Lin Tong
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Insects. 2020 Apr 15;11(4):245. doi: 10.3390/insects11040245.
Autophagy is a highly conserved and regulated process in eukaryotic cells and remodels cytoplasm, recovers essential nutrients, and disposes of unwanted cytoplasmic components. Autophagy-related gene (ATG) 8, identified in Moore, which is an oligophagous pest of (Lour.), was characterized (). Multiple sequence alignment showed that possesses highly conserved domain structures. Stage- and tissue-specific expressions indicated that is highly expressed in prepupal, pupal, and adult stages and in the midgut of larvae and abdomen of adults. Lack of function of by RNA interference resulted in a significant decrease in survival rate and an increase in abnormal or nonviable phenotypes in . . Transition rate from larval to pupal stages was 33.0% and from pupal to adult stages was 15.0% after injection. Reduction of ATG8 expression reduced survival of . . Therefore, possibly plays a key role in normal growth stage of . . suppression downregulates expression, suggesting that the two genes are interconnected. Further, expression increased by 20-hydroxyecdysone treatment, starvation, and extreme temperature exposure. Starvation also altered expression of other ATGs in . . This study may be used to guide research on molecular mechanisms of autophagy in insects.
自噬是真核细胞中一个高度保守且受调控的过程，它重塑细胞质、回收必需营养物质并清除不需要的细胞质成分。在 Moore 中鉴定出的自噬相关基因（ATG）8，Moore 是 （Lour.）的一种寡食性害虫，其特性已得到表征（）。多序列比对表明 具有高度保守的结构域。阶段和组织特异性表达表明 在蛹前期、蛹期和成虫期以及幼虫中肠和成虫腹部高度表达。通过 RNA 干扰使 功能缺失导致 存活率显著下降以及异常或无活力表型增加。注射后从幼虫到蛹期的转化率为 33.0%，从蛹到成虫期的转化率为 15.0%。ATG8 表达降低降低了 的存活率。因此， 可能在 的正常生长阶段起关键作用。 抑制下调了 表达，表明这两个基因相互关联。此外，20-羟基蜕皮酮处理、饥饿和极端温度暴露会使 表达增加。饥饿也改变了 中其他 ATG 的表达。本研究可用于指导昆虫自噬分子机制的研究。
