Liu Jialin, Dong Xiaoke, Li Zhonghao, Wang Gesheng, Zhou Yujia, Liu Chongchong, Wang Kaiyue, Li Lili, Liu Jinmin
Department of Neurosurgery, Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, China.
Department of Neurology, Dongfang Hospital Beijing University of Chinese Medicine, Beijing 100078, China.
Evid Based Complement Alternat Med. 2020 Apr 6;2020:2305017. doi: 10.1155/2020/2305017. eCollection 2020.
(XYK) is a Chinese patent medicine approved by the National Medical Product Administration which is used to treat intracranial hematoma in China. In this study, we observed the molecular mechanism of XYK in hypoxia-inducible factor 1 (HIF-1), inflammation and angiogenesis of chronic subdural hematoma (CSDH). The CSDH model was made by using internal iliac vein blood of Wister rats, and rats were divided into sham group, CSDH group and XYK group. The rats in the XYK group were gavaged with (185 mg/kg) for 7 days, and rats in the CSDH group and sham group were gavaged with the same amount of physiological saline for 7 days. In the CSHD rat model, active inflammation and angiogenesis were observed around the hematoma. XYK promoted the ubiquitination and degradation of HIF-1, and reduced the concentration of VEGF and the ratio of angiopoietin-1/angiopoietin-2. XYK reduced proinflammatory cytokines and increased anti-inflammatory cytokine. In tissue section, XYK reduced the size of the hematoma and membrane, and reduced vWF positive cells in membrane. Furthermore, the endothelial progenitor cells in blood decreased as well. Overall, XYK shows anti-inflammatory and antiangiogenesis effects which may relate to the degradation of HIF-1.
醒脑再造胶囊（XYK）是一种经国家药品监督管理局批准的用于治疗颅内血肿的中成药。在本研究中，我们观察了醒脑再造胶囊对慢性硬膜下血肿（CSDH）缺氧诱导因子1（HIF-1）、炎症和血管生成的分子机制。采用Wister大鼠髂内静脉血制作CSDH模型，并将大鼠分为假手术组、CSDH组和醒脑再造胶囊组。醒脑再造胶囊组大鼠给予185mg/kg药物灌胃7天，CSDH组和假手术组大鼠给予等量生理盐水灌胃7天。在CSDH大鼠模型中，血肿周围观察到活跃的炎症和血管生成。醒脑再造胶囊促进HIF-1的泛素化和降解，降低血管内皮生长因子（VEGF）浓度和血管生成素-1/血管生成素-2的比值。醒脑再造胶囊降低促炎细胞因子水平，增加抗炎细胞因子水平。在组织切片中，醒脑再造胶囊减小了血肿和包膜的大小，并减少了包膜中血管性血友病因子（vWF）阳性细胞。此外，血液中的内皮祖细胞也减少了。总体而言，醒脑再造胶囊具有抗炎和抗血管生成作用，这可能与HIF-1的降解有关。
