Clarke P G, Egloff M
Institute of Anatomy, University of Lausanne, Switzerland.
Anat Embryol (Berl). 1988;179(2):103-8. doi: 10.1007/BF00304692.
We have studied the effects on the chick embryo's isthmo-optic nucleus of de-efferentation alone or in combination with deafferentation. De-efferentation was achieved by pharmacological destruction of the axonal target cells in the retina at E13, or by colchicine-blockade of axoplasmic transport in the intraocular parts of the isthmo-optic axons at E13; deafferentation was by a tectal lesion at E11 or E12. De-efferentation alone causes all the isthmo-optic neurons to die, and mostly by the "endocytic-autophagic" mode of cell death, which is characterized by pronounced endocytosis (of an intravascularly injected label) and by intense, clumped activity of two lysosomal enzymes (acid phosphatase and N-acetyl-beta-glucosaminidase). Deafferentation plus de-efferentation caused there to be less endocytic-autophagic dying cells in the isthmo-optic nucleus than after de-efferentation alone, but all the neurons still died. Our interpretation is that deafferentation switched many of the isthmo-optic neurons to a completely different (nonendocytic, nonautophagic) mode of cell death.
我们研究了单独去传入或联合去传入对鸡胚峡视核的影响。去传入是通过在胚胎第13天对视网膜中的轴突靶细胞进行药理学破坏，或通过在胚胎第13天用秋水仙碱阻断峡视轴突眼内部分的轴浆运输来实现；去传入是通过在胚胎第11天或第12天进行顶盖损伤来实现。单独去传入会导致所有峡视神经元死亡，且大多通过“内吞-自噬”细胞死亡模式，其特征是明显的内吞作用（对血管内注射标记物的内吞）以及两种溶酶体酶（酸性磷酸酶和N-乙酰-β-葡萄糖苷酶）的强烈、聚集性活性。去传入加去传入导致峡视核中内吞-自噬死亡细胞比单独去传入后更少，但所有神经元仍然死亡。我们的解释是，去传入使许多峡视神经元转变为完全不同的（非内吞、非自噬）细胞死亡模式。
