P. K. Anokhin Research Institute of Normal Physiology, Moscow, Russia.
A. I. Evdokimov Moscow State University of Medicine and Dentistry, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2020 Apr;168(6):713-717. doi: 10.1007/s10517-020-04786-w. Epub 2020 Apr 23.
We studied changes in the blood cytokine profile of rats 3 h, 1 day, and 8 days after acute stress on the model of 24-h immobilization followed by LPS administration (100 μg/kg intraperitoneally). The concentration of proinflammatory cytokines (particularly of IL-1β and TNFα) significantly decreased at the early stage after stress exposure and physiological saline injection, but increased in the follow-up period and practically did not differ or even surpassed the control level by the end of observations. Under these conditions, the blood content of anti-inflammatory cytokine IL-10 increased most significantly on day 1 of the post-stress period. Restraint stress followed by LPS administration was accompanied by a decrease in the level of proinflammatory cytokines at the early (IFNγ and TNFα) and late stages (IL-1β) of the experiment. Directed modulation of the immune status in animals after acute stress was followed by a significant increase in the content of IL-10 on days 1 and 8, as well as by a tendency toward elevation of IL-4 concentration by the end of the study. The directionality and degree of changes in the cytokine profile of mammalian tissues depend on the type of extreme exposure, duration of the post-stress period, and specific effects of exogenous pathogenic factors in the whole body.
我们研究了急性应激后 3 小时、1 天和 8 天的大鼠血液细胞因子谱的变化，应激模型为 24 小时束缚后给予 LPS（100μg/kg 腹腔内注射）。在应激暴露和生理盐水注射后的早期，促炎细胞因子（尤其是 IL-1β 和 TNFα）的浓度显著降低，但在随后的时间内增加，实际上在观察结束时并未降低或甚至超过对照水平。在这些条件下，抗炎细胞因子 IL-10 的血液含量在应激后第 1 天增加最显著。束缚应激后给予 LPS 会导致实验早期（IFNγ 和 TNFα）和晚期（IL-1β）促炎细胞因子水平降低。急性应激后动物的免疫状态定向调节后，IL-10 的含量在第 1 天和第 8 天显著增加，研究结束时 IL-4 浓度也有升高的趋势。哺乳动物组织细胞因子谱的变化方向和程度取决于极端暴露的类型、应激后时期的持续时间以及全身外源性致病因素的特定影响。
