Department of Chemistry, Hacettepe University, Ankara, Turkey.
Biotechnol Prog. 2020 Sep;36(5):e3006. doi: 10.1002/btpr.3006. Epub 2020 May 8.
Molecularly imprinted polymers are used for creating a specific cavity and selective recognition sites for the structure of a target molecule in a polymeric structure. In this study, specific molecularly imprinted cryogel cartridges were synthesized using two distinct functional monomers to compare imprinting efficiency for the selective recognition of Tyrosine (Tyr). Tyr-imprinted cryogel cartridge (MIP1) was prepared using metal-chelate coordination for the imprinting process by free-radical bulk polymerization under frozen conditions, and Tyr-imprinted cryogel cartridge (MIP2) was prepared in the same way using hydrophobic effects for imprinting. After the characterization of the cryogel cartridges was carried out, the optimum adsorption conditions of both were determined according to the different parameters such as flow rate (0.5-2.5 ml/min), pH of the medium (4.0-8.0), initial Tyr concentration (0.1-3.0 mg/ml), and temperature (4-45°C). Selectivity experiments of Tyr-imprinted and non-imprinted cryogel cartridges were carried out by using phenylalanine, tryptophan, and cysteine. Besides, the eluted Tyr from MIP1 and MIP2 cryogel cartridge were applied to FPLC system. Also, the reusability experiments of Tyr-imprinted cryogel cartridges was observed no significant decrease in the adsorption capacity.
分子印迹聚合物用于在聚合物结构中为目标分子的结构创建特定的腔和选择性识别位点。在这项研究中，使用两种不同的功能单体合成了特定的分子印迹冷冻凝胶筒，以比较用于选择性识别酪氨酸（Tyr）的印迹效率。Tyr 印迹冷冻凝胶筒（MIP1）通过自由基本体聚合在冷冻条件下通过金属螯合配位进行印迹过程制备，而 Tyr 印迹冷冻凝胶筒（MIP2）以同样的方式使用疏水性效应进行印迹。在对冷冻凝胶筒进行表征后，根据不同的参数（流速（0.5-2.5 ml/min）、介质 pH（4.0-8.0）、初始 Tyr 浓度（0.1-3.0 mg/ml）和温度（4-45°C））确定了两者的最佳吸附条件。通过使用苯丙氨酸、色氨酸和半胱氨酸对 Tyr 印迹和非印迹冷冻凝胶筒进行了选择性实验。此外，从 MIP1 和 MIP2 冷冻凝胶筒中洗脱的 Tyr 被应用于 FPLC 系统。此外，还观察到 Tyr 印迹冷冻凝胶筒的可重复使用实验中吸附容量没有明显下降。
