Key Laboratory of Dairy Science, Northeast Agricultural University, College of Food Science, Harbin 150030, People's Republic of China.
Food Funct. 2020 May 1;11(5):3823-3837. doi: 10.1039/c9fo02992c. Epub 2020 Apr 24.
Ulcerative colitis (UC) is a common inflammatory bowel disease (IBD) that has serious harmful effects on human health. Lactobacillus rhamnosus, a probiotic, has a strong colonization and adhesion effect and improves the intestinal health of the host. Inulin has good anti-inflammatory effects and can promote the proliferation of beneficial intestinal bacteria. The purpose of this study was to investigate the alleviating effects of L. rhamnosus 1.0320 in combination with inulin on UC, examining the resulting changes in intestinal flora. A UC model was established by having mice freely drink a 3% (w/v) dextran sodium sulphate (DSS) solution for seven days. After successful modeling, the mice were given antibiotics, L. rhamnosus 1.0320 by itself, inulin by itself, and L. rhamnosus 1.0320 combined with inulin as an intragastric intervention for 28 days. The abundance and structural changes of bacteria in the intestinal content of mice were analyzed by 16S rDNA high-throughput sequencing. The study found that male BALB/c mice can successfully establish a typical model of small intestinal inflammation by freely drinking a 3% DSS solution for one week. L. rhamnosus 1.0320 combined with inulin can alleviate DSS-induced colitis, reduce the Disease Activity Index (DAI) score of the pathological damage of colon tissue, decrease myeloperoxidase (MPO) activity, increase hemoglobin content, and regulate the expression levels of inflammatory cytokines IL-1β, IL-6, TNF-α and IL-10. The intestinal flora of mice is reduced after enteritis, and its structure gets disordered. The combination of L. rhamnosus 1.0320 and inulin can increase the abundance and diversity of intestinal flora, and increase the content of beneficial bacteria. Prebiotics promote the colonization ability of probiotics. L. rhamnosus 1.0320 combined with inulin can change the intestinal flora to relieve ulcerative colitis, providing a new theoretical basis for the study of UC mechanism.
溃疡性结肠炎（UC）是一种常见的炎症性肠病（IBD），对人类健康有严重的危害。鼠李糖乳杆菌是一种益生菌，具有较强的定植和黏附作用，能改善宿主的肠道健康。菊粉具有良好的抗炎作用，并能促进有益肠道细菌的增殖。本研究旨在探讨鼠李糖乳杆菌 1.0320 与菊糖联合应用对 UC 的缓解作用，观察肠道菌群的变化。通过让小鼠自由饮用 3%（w/v）葡聚糖硫酸钠（DSS）溶液 7 天建立 UC 模型。建模成功后，用抗生素、鼠李糖乳杆菌 1.0320 单独、菊糖单独和鼠李糖乳杆菌 1.0320 联合菊糖进行灌胃干预 28 天。通过 16S rDNA 高通量测序分析小鼠肠道内容物中细菌的丰度和结构变化。研究发现，雄性 BALB/c 小鼠通过自由饮用 3% DSS 溶液 1 周即可成功建立小肠炎症的典型模型。鼠李糖乳杆菌 1.0320 联合菊糖可缓解 DSS 诱导的结肠炎，降低结肠组织病理损伤的疾病活动指数（DAI）评分，降低髓过氧化物酶（MPO）活性，增加血红蛋白含量，调节炎症细胞因子 IL-1β、IL-6、TNF-α和 IL-10 的表达水平。肠炎后小鼠肠道菌群减少，结构紊乱。鼠李糖乳杆菌 1.0320 联合菊糖能增加肠道菌群的丰度和多样性，增加有益菌的含量。益生菌的定植能力可以促进益生菌的定植。鼠李糖乳杆菌 1.0320 联合菊糖可以改变肠道菌群，缓解溃疡性结肠炎，为 UC 机制的研究提供了新的理论依据。
