Arguelles J C, Gacto M
Department of Microbiology, Facultad de Biología, Universidad de Murcia, Spain.
Antonie Van Leeuwenhoek. 1988;54(6):555-65. doi: 10.1007/BF00588391.
The isolation of vacuoles by density gradient centrifugation of protoplast lysates from Candida utilis cells showed a high specific activity for nonregulatory trehalase in vacuoles whereas the regulatory trehalase activatable by phosphorylation behaves as a cytoplasmic enzyme. The vacuolar trehalase is a glycoprotein that can be precipitated by Con A-Sepharose. Treatment of this enzyme with endo H reduced its reactivity with the lectin without loss of enzyme activity and decreased its apparent molecular weight by gel filtration.
