从印度牛中分离和鉴定牛疱疹病毒 5（BoHV5）。
Isolation and characterization of bovine herpes virus 5 (BoHV5) from cattle in India.
机构信息
National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, India.
出版信息
PLoS One. 2020 Apr 24;15(4):e0232093. doi: 10.1371/journal.pone.0232093. eCollection 2020.
Bovine herpesvirus 1 (BoHV1) and 5 (BoHV5) are genetically and antigenically related alphaherpesviruses. Infection with one virus induces protective immunity against the other. However, disease associated with BoHV1 and BoHV5 varies significantly; whereas BoHV1 infection is usually associated with rhinotracheitis and abortion, BoHV5 causes encephalitis in cattle. BoHV5 outbreaks are sporadic and mainly restricted to the South American countries. We report BoHV5 infection for the first time from aborted cattle in India. Based on the characteristic cytopathic effects in MDBK cells, amplification of the viral genome by PCR, differential PCR for BoHV1/BoHV5, nucleotide sequencing and restriction endonuclease patterns, identity of the virus was confirmed as BoHV5 subtype A. Serum samples from the aborted cattle strongly neutralized both BoHV1 and BoHV5 suggesting an active viral infection in the herd. Upon UL27, UL44 and UL54 gene-based sequence and phylogenetic analysis, the isolated virus clustered with BoHV5 strains and showed highest similarity with the Brazilian BoHV5 strains.
牛疱疹病毒 1 型（BoHV1）和 5 型（BoHV5）是具有遗传和抗原相关性的α疱疹病毒。感染其中一种病毒可诱导对另一种病毒的保护性免疫。然而，与 BoHV1 和 BoHV5 相关的疾病有很大的差异；BoHV1 感染通常与鼻气管炎和流产有关，而 BoHV5 则会导致牛脑炎。BoHV5 的爆发是零星的，主要局限于南美洲国家。我们首次报告了印度流产牛的 BoHV5 感染。根据 MDBK 细胞中的特征性细胞病变效应、PCR 扩增病毒基因组、BoHV1/BoHV5 的差异 PCR、核苷酸测序和限制性内切酶图谱，确认该病毒为 BoHV5 亚 A 型。来自流产牛的血清样本强烈中和了 BoHV1 和 BoHV5，表明该牛群中存在活跃的病毒感染。基于 UL27、UL44 和 UL54 基因的序列和系统发育分析，分离出的病毒与 BoHV5 株聚类，并与巴西 BoHV5 株显示出最高的相似性。
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