鲭鱼（日本鲭）肌肉蛋白水解物中新型抗氧化肽的鉴定与表征
Identification and characterization of novel antioxidant peptides from mackerel (Scomber japonicus) muscle protein hydrolysates.
作者信息
Bashir Khawaja Muhammad Imran, Sohn Jae Hak, Kim Jin-Soo, Choi Jae-Suk
机构信息
German Engineering Research Center for Life Science Technologies in Medicine and Environment, 31, Gwahaksan 1-ro, 60 Bean-gil, Gangseo-gu, Busan 46742, Republic of Korea; Seafood Research Center, IACF, Silla University, 606, Advanced Seafood Processing Complex, Wonyang-ro, Amnam-dong, Seo-gu, Busan 49277, Republic of Korea.
Seafood Research Center, IACF, Silla University, 606, Advanced Seafood Processing Complex, Wonyang-ro, Amnam-dong, Seo-gu, Busan 49277, Republic of Korea; Department of Food Biotechnology, Division of Bioindustry, College of Medical and Life Sciences, Silla University, 140, Baegyang-daero 700 beon-gil, Sasang-gu, Busan 46958, Republic of Korea.
出版信息
Food Chem. 2020 Apr 15;323:126809. doi: 10.1016/j.foodchem.2020.126809.
Antioxidant peptides are commonly used as functional ingredient in the pharmaceutical industries. Here, we characterized the antioxidant peptides from mackerel muscle protein hydrolysates (MPHs). MPHs showing higher bioactivities were separated into seven groups by FPLC. MPH-3 which exhibited significantly higher (p < 0.05) DPPH scavenging activity (32.12 ± 3.01%) was fractionated using RP-HPLC to obtain purified fractions A and B, which were further subjected to MALDI-TOF/TOF-MS for mass fingerprinting. Fraction A exhibited the highest (p < 0.05) DPPH scavenging activity (34.11 ± 1.52%), and it contained 21 peptides characterized by LC-MS/MS-. Ten peptides were synthesized, and their antioxidant activities were evaluated; one of the peptides, ALSTWTLQLGSTSFSASPM, showed the highest (p < 0.05) DPPH scavenging activity (36.34 ± 4.64%) and another peptide, LGTLLFIAIPI, exhibited the highest (p < 0.05) SOD-like activity (28.94 ± 4.19%). The results of this study indicate that MPHs could serve as a suitable source of antioxidant peptides.
抗氧化肽通常用作制药行业的功能成分。在此，我们对鲭鱼肌肉蛋白水解物（MPHs）中的抗氧化肽进行了表征。通过快速蛋白质液相色谱（FPLC）将具有较高生物活性的MPHs分为七组。使用反相高效液相色谱（RP-HPLC）对表现出显著更高（p < 0.05）二苯基苦味酰基自由基（DPPH）清除活性（32.12 ± 3.01%）的MPH-3进行分级分离，以获得纯化级分A和B，进一步对其进行基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF-MS）分析以进行质量指纹识别。级分A表现出最高（p < 0.05）的DPPH清除活性（34.11 ± 1.52%），并且它包含通过液相色谱-串联质谱（LC-MS/MS）鉴定的21种肽。合成了十种肽，并评估了它们的抗氧化活性；其中一种肽ALSTWTLQLGSTSFSASPM表现出最高（p < 0.05）的DPPH清除活性（36.34 ± 4.64%），另一种肽LGTLLFIAIPI表现出最高（p < 0.05）的超氧化物歧化酶（SOD）样活性（28.94 ± 4.19%）。本研究结果表明，MPHs可作为抗氧化肽的合适来源。
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