Wareth Gamal, Pletz Mathias W, Neubauer Heinrich, Murugaiyan Jayaseelan
Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.
Institute for Infectious Diseases and Infection Control, Jena University Hospital, Am Klinikum 1, 07747 Jena, Germany.
Microorganisms. 2020 May 20;8(5):766. doi: 10.3390/microorganisms8050766.
Brucellosis is a global zoonosis caused by Gram-negative, facultative intracellular bacteria of the genus Brucella (B.). Proteomics has been used to investigate a few B. melitensis and B. abortus strains, but data for other species and biovars are limited. Hence, a comprehensive analysis of proteomes will significantly contribute to understanding the enigmatic biology of brucellae. For direct identification and typing of Brucella, matrix-assisted laser desorption ionization - time of flight mass spectrometry (MALDI - TOF MS) has become a reliable tool for routine diagnosis due to its ease of handling, price and sensitivity highlighting the potential of proteome-based techniques. Proteome analysis will also help to overcome the historic but still notorious Brucella obstacles of infection medicine, the lack of safe and protective vaccines and sensitive serologic diagnostic tools by identifying the most efficient protein antigens. This perspective summarizes past and recent developments in Brucella proteomics with a focus on species identification and serodiagnosis. Future applications of proteomics in these fields are discussed.
布鲁氏菌病是一种由革兰氏阴性兼性胞内菌布鲁氏菌属引起的全球人畜共患病。蛋白质组学已被用于研究少数羊种布鲁氏菌和牛种布鲁氏菌菌株，但关于其他种和生物变种的数据有限。因此，对蛋白质组进行全面分析将极大地有助于理解布鲁氏菌神秘的生物学特性。对于布鲁氏菌的直接鉴定和分型，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）因其操作简便、价格低廉和灵敏度高，突出了基于蛋白质组技术的潜力，已成为常规诊断的可靠工具。蛋白质组分析还将有助于克服感染医学中历史悠久但仍然声名狼藉的布鲁氏菌障碍，即缺乏安全有效的疫苗和敏感的血清学诊断工具，通过鉴定最有效的蛋白质抗原。本综述总结了布鲁氏菌蛋白质组学的过去和近期进展，重点关注菌种鉴定和血清学诊断。并讨论了蛋白质组学在这些领域的未来应用。
