Schneider Vanessa Suzane, Bark Juliana Müller, Winnischofer Sheila M B, Dos Santos Elisvânia Freitas, Iacomini Marcello, Cordeiro Lucimara M C
Postgraduate Program in Biochemistry Sciences, Sector of Biological Sciences, Federal University of Paraná, Curitiba, PR, 81531-990, Brazil.
Postgraduate Program in Biochemistry Sciences, Sector of Biological Sciences, Federal University of Paraná, Curitiba, PR, 81531-990, Brazil; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba, Brazil; Postgraduate Program in Cell and Molecular Biology, Federal University of Paraná, Curitiba, Brazil.
Food Res Int. 2020 Jun;132:109065. doi: 10.1016/j.foodres.2020.109065. Epub 2020 Feb 4.
Exotic fruits and their co-products may be valuable sources of antioxidant dietary fibres (DF) which are useful for food industry and human health. In this study, we aimed to characterize DF obtained from guavira fruit pomace and investigate its antioxidant potential employing TEAC assay as well as a cell model. The DF were chemically characterized as containing arabinan, highly-methoxylated homogalacturonan and arabinogalactan. The DF-containing fraction (CPW) presented ABTS free radical scavenger activity. MTT and DCFH-DA assay were performed to assess, respectively, changes in cell viability and the potential intracellular antioxidant activity against HO-induced oxidative stress in murine NIH 3T3 fibroblast. CPW exhibited no effects on cell viability, moreover, when administered 48 h prior the induction of HO toxic effects, it protected the cells, significantly increasing the cell viability compared to control. This protection may be related to the observed reduction of reactive oxygen species levels. Thus, the pre-treatment of cells with guavira DF for 48 h remarkably induced a cytoprotection against pro-oxidant conditions, and may be a valuable functional compound recovered from an unexploited agroindutrial waste.
外来水果及其副产品可能是抗氧化膳食纤维（DF）的宝贵来源，这些膳食纤维对食品工业和人类健康有益。在本研究中，我们旨在对从番樱桃果渣中获得的膳食纤维进行表征，并采用TEAC测定法以及细胞模型研究其抗氧化潜力。这些膳食纤维经化学表征含有阿拉伯聚糖、高甲氧基化同型半乳糖醛酸聚糖和阿拉伯半乳聚糖。含膳食纤维部分（CPW）具有ABTS自由基清除活性。进行MTT和DCFH-DA测定，分别评估细胞活力的变化以及对小鼠NIH 3T3成纤维细胞中由HO诱导的氧化应激的潜在细胞内抗氧化活性。CPW对细胞活力没有影响，此外，在诱导HO毒性作用前48小时给予CPW，它对细胞起到了保护作用，与对照组相比显著提高了细胞活力。这种保护作用可能与观察到的活性氧水平降低有关。因此，用番樱桃膳食纤维预处理细胞48小时可显著诱导细胞对促氧化条件的保护作用，并且可能是一种从未开发的农业工业废料中回收的有价值的功能性化合物。
