UMR 1253, iBrain, Université de Tours, Inserm, Tours, France.
Laboratory of Veterinary Internal Medicine, Department of Clinical Sciences; Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Ultrasound Med Biol. 2020 Jul;46(7):1565-1583. doi: 10.1016/j.ultrasmedbio.2020.03.013. Epub 2020 Apr 22.
Microbubble-assisted ultrasound has emerged as a promising method for local drug delivery. Microbubbles are intravenously injected and locally activated by ultrasound, thus increasing the permeability of vascular endothelium for facilitating extravasation and drug uptake into the treated tissue. Thereby, endothelial cells are the first target of the effects of ultrasound-driven microbubbles. In this review, the in vitro and in vivo bioeffects of this method on endothelial cells are described and discussed, including aspects on the permeabilization of biologic barriers (endothelial cell plasma membranes and endothelial barriers), the restoration of their integrity, the molecular and cellular mechanisms involved in both these processes, and the resulting intracellular and intercellular consequences. Finally, the influence of the acoustic settings, microbubble parameters, treatment schedules and flow parameters on these bioeffects are also reviewed.
微泡辅助超声已成为一种很有前途的局部药物输送方法。微泡通过静脉注射并在局部被超声激活，从而增加血管内皮的通透性，促进外渗和药物进入治疗组织。因此，内皮细胞是超声驱动微泡作用的第一个靶标。在这篇综述中，描述并讨论了该方法对内皮细胞的体外和体内生物效应，包括对生物屏障（内皮细胞的质膜和内皮屏障）通透性的影响、其完整性的恢复、这两个过程中涉及的分子和细胞机制，以及由此产生的细胞内和细胞间后果。最后，还回顾了声参数、微泡参数、治疗方案和流参数对这些生物效应的影响。
