一种用于检测尿白细胞酯酶活性的简单分光光度测定法。

A simple spectrophotometric assay for urinary leukocyte esterase activity.

作者信息

Murthy V V, Karmen A

机构信息

Department of Laboratory Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.

出版信息

Biochem Med Metab Biol. 1988 Dec;40(3):260-8. doi: 10.1016/0885-4505(88)90127-2.

DOI:10.1016/0885-4505(88)90127-2

PMID:3233183

Abstract

We have developed a sensitive spectrophotometric method for assaying urinary leukocyte esterase activity by employing a synthetic substrate, N-toluene sulfonyl indoxyl alanine ester. This kinetic assay can be performed with a small aliquot of urine, by following the change in absorbance of the chromophore at 385 nm. It is rapid and specific for leukocyte esterase and therefore can be used in the early diagnosis of urinary tract infection.

摘要

我们开发了一种灵敏的分光光度法，通过使用合成底物N-甲苯磺酰吲哚丙氨酸酯来测定尿白细胞酯酶活性。这种动力学测定法可以用少量尿液进行，通过跟踪发色团在385nm处吸光度的变化来实现。它对白细胞酯酶快速且具有特异性，因此可用于尿路感染的早期诊断。