使用净速率常数的稳态酶动力学通用方程及其在过氧化氢酶反应动力学分析中的应用。
General equation of steady-state enzyme kinetics using net rate constants and its applicaiton to the kinetic analysis of catalase reaction.
作者信息
Yomo T, Yamano T, Yamamoto K, Urabe I
机构信息
Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.
出版信息
J Theor Biol. 1997 Oct 7;188(3):301-12. doi: 10.1006/jtbi.1997.0474.
The steady-state velocity equation is derived for the general reaction scheme containing n kinds of enzyme species connected by a network of reversible reaction steps. The general equation is represented by the net rate constants as well as the true rate constants of the individual reaction steps. Using a general equation, equations for simpler schemes can easily be derived. Furthermore, the velocity equation expressed by net rate constants is useful for understanding the dynamic state of any reaction be it simple or complex. The generality of the presented equation is tested in a complex reaction involving Bacillus stearothermophilus catalase I. In addition, the applicability of the general equation in analysing the reaction specificity and dynamic state of the reaction is shown.
针对由可逆反应步骤网络连接的包含n种酶物种的一般反应体系，推导了稳态速度方程。该一般方程由净速率常数以及各个反应步骤的真实速率常数表示。利用这个一般方程，可以很容易地推导出更简单反应体系的方程。此外，用净速率常数表示的速度方程有助于理解任何反应（无论简单还是复杂）的动态状态。在涉及嗜热脂肪芽孢杆菌过氧化氢酶I的复杂反应中检验了所提出方程的通用性。此外，还展示了该一般方程在分析反应特异性和反应动态状态方面的适用性。
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