一种用于蛋白质定量的灵敏且快速的免疫化学方法。

A sensitive and rapid immunochemical method for quantitation of proteins.

作者信息

Mawal M R, Mawal Y R, Ranadive S N, Ranjekar P K

机构信息

Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.

出版信息

Biosci Rep. 1988 Oct;8(5):427-33. doi: 10.1007/BF01121640.

DOI:10.1007/BF01121640

PMID:3233341

Abstract

A sensitive and rapid ELISA for quantitation of seed globulins is described. This method employs conjugation of pigeon pea (Cajanus cajan) globulin antibodies and the enzyme peroxidase together with dextran. Using this conjugate, proteins as low as 0.1 ng were detected. Dextran conjugate has a ten-fold greater efficiency of quantitating pigeon pea globulins than the commercial goat anti-rabbit IgG conjugate, and is three-fold more efficient than pigeon pea globulin IgG peroxidase conjugate. The method can be conveniently adapted for quantitation of other proteins also.

摘要

本文描述了一种用于定量种子球蛋白的灵敏且快速的酶联免疫吸附测定法（ELISA）。该方法采用木豆（Cajanus cajan）球蛋白抗体与酶过氧化物酶以及葡聚糖进行偶联。使用这种偶联物，可检测低至0.1纳克的蛋白质。葡聚糖偶联物在定量木豆球蛋白方面的效率比市售山羊抗兔IgG偶联物高十倍，比木豆球蛋白IgG过氧化物酶偶联物高三倍。该方法也可方便地用于其他蛋白质的定量。