Wongyai S, Oefner P, Bonn G
Institute of Radiochemistry, University of Innsbruck, Austria.
Biomed Chromatogr. 1988 Nov;2(6):254-7. doi: 10.1002/bmc.1130020606.
Analytical methods are described for the quantitative determination of putrescine, cadaverine, spermidine, spermine and the acetylated derivatives of spermidine and spermine in biological fluids using pre-column derivatization with either benzoyl chloride or 3,5-dinitrobenzoyl chloride, which were added to each sample as solutions in diethyl ether. Putrescine, spermidine and spermine can be analysed in seminal plasma at nanogram levels when benzoyl chloride is used as derivatizing agent. In the analysis of putrescine, cadaverine, spermidine and acetyl derivatives of spermidine and spermine, higher sensitivity is obtained with 3,5-dinitrobenzoyl-chloride. This method can readily be used in the determination of acetylated polyamines in urine samples.
描述了使用苯甲酰氯或3,5-二硝基苯甲酰氯进行柱前衍生化，对生物体液中的腐胺、尸胺、亚精胺、精胺以及亚精胺和精胺的乙酰化衍生物进行定量测定的分析方法，这些试剂以乙醚溶液的形式添加到每个样品中。当使用苯甲酰氯作为衍生剂时，精液中的腐胺、亚精胺和精胺可以在纳克水平进行分析。在分析腐胺、尸胺、亚精胺以及亚精胺和精胺的乙酰化衍生物时，使用3,5-二硝基苯甲酰氯可获得更高的灵敏度。该方法可轻松用于尿液样品中乙酰化多胺的测定。
