Aboul-Enein H Y, al-Duraibi I A
Biological and Medical Research Department (MBC 03), King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
Biomed Chromatogr. 1998 Sep-Oct;12(5):291-3. doi: 10.1002/(SICI)1099-0801(199809/10)12:5<291::AID-BMC749>3.0.CO;2-4.
Free polyamines and their acetylated derivatives, [putrescine (Put), spermine (Spm), spermidine (Spd), N1-acetylspermidine N1-AcSpd), N8-acetylspermidine N8-AcSpd)], were separated using ion-pair reversed-phase liquid chromatography after pre-column derivatization with dansyl chloride. Also 1,6-hexanediamine (1,6-Hda) and 1,7-diaminoheptane (1,7-Dah) which used as an internal standards were separated under the same chromatographic conditions. The reported method provided a good resolution between N1-AcSpd and N8 AcSpd (Rs = 0.86). This quality of resolution together with the very low detection limit obtained, (33.6, 8.33, 2.72, 2.67, 4.76, 4.27, and 2.27 fmoles/microliters for N1-AcSpd, N8-AcSpd, Put, 1,6-Hda, 1,7-Dah, Spm, and Spd, respectively), offer advantages to this method. Furthermore, a significant correlation coefficients were obtained as follows: 0.998 (N8-AcSpd), 0.996 N1-AcSpd), 0.997 (Put), 0.9995 (1,6-Hda), 0.9992 (1,7-Dah), 0.9996 (Spd), and 0.997 (Spm). The described method can be adapted for the separation of the free and acetylated polyamines in biological specimens.
游离多胺及其乙酰化衍生物（腐胺（Put）、精胺（Spm）、亚精胺（Spd）、N1-乙酰亚精胺（N1-AcSpd）、N8-乙酰亚精胺（N8-AcSpd））在经丹磺酰氯柱前衍生后，采用离子对反相液相色谱法进行分离。用作内标的1,6-己二胺（1,6-Hda）和1,7-二氨基庚烷（1,7-Dah）也在相同的色谱条件下进行分离。所报道的方法在N1-AcSpd和N8-AcSpd之间提供了良好的分离度（Rs = 0.86）。这种分离度以及所获得的极低检测限（N1-AcSpd、N8-AcSpd、Put、1,6-Hda、1,7-Dah、Spm和Spd的检测限分别为33.6、8.33、2.72、2.67、4.76、4.27和2.27飞摩尔/微升）为该方法带来了优势。此外，还获得了如下显著的相关系数：0.998（N8-AcSpd）、0.996（N1-AcSpd）、0.997（Put）、0.9995（1,6-Hda）、0.9992（1,7-Dah）、0.9996（Spd）和0.997（Spm）。所描述的方法可适用于生物样本中游离和乙酰化多胺的分离。
