Department of Physiology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.
Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Life Sci. 2020 Jul 15;253:117703. doi: 10.1016/j.lfs.2020.117703. Epub 2020 Apr 22.
Vitamin D is a well-known endocrine regulator of calcium/phosphate homeostasis and has been reported as having a wide range of activities that are potentially beneficial for human health. This study aimed to investigate the effects of pretreatment of vitamin D (100, 1000, and 10,000 IU/kg) against lipopolysaccharide (LPS)-induced cognitive impairment in rats.
Male Wistar rats were divided into five groups. The passive avoidance test and Morris water maze (MWM) test were conducted to evaluate the learning and memory function. Oxidative stress markers including malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), total thiol content as well as interleukin (IL)-6 were evaluated in the hippocampus tissue.
The intraperitoneal (i.p.) injection of LPS (1 mg/kg) correlates with deficits in passive avoidance and spatial learning in the systemic inflammation model. However, pretreatment with vitamin D improved LPS-induced cognitive impairment. In addition, vitamin D decreased IL-6 and MDA levels, whereas the activities of CAT, SOD, and total thiol content in the hippocampus tissue were significantly increased.
In conclusion, our results suggest that vitamin D plays a protective role against memory dysfunction caused by LPS-induced inflammation through inhibition of oxidative stress and inflammation in the hippocampus. Vitamin D may be a promising potential therapeutic supplement for the treatment or prevention of learning and memory disorders.
维生素 D 是一种众所周知的钙/磷代谢内分泌调节剂，据报道，它具有广泛的潜在有益人类健康的活性。本研究旨在探讨维生素 D（100、1000 和 10000IU/kg）预处理对脂多糖（LPS）诱导的大鼠认知功能障碍的影响。
雄性 Wistar 大鼠分为五组。通过被动回避试验和 Morris 水迷宫（MWM）试验评估学习和记忆功能。评估海马组织中的氧化应激标志物，包括丙二醛（MDA）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、总巯基含量和白细胞介素（IL）-6。
腹腔内（i.p.）注射 LPS（1mg/kg）与全身炎症模型中被动回避和空间学习能力下降相关。然而，维生素 D 预处理可改善 LPS 诱导的认知障碍。此外，维生素 D 降低了 IL-6 和 MDA 水平，而海马组织中 CAT、SOD 和总巯基含量的活性显著增加。
总之，我们的结果表明，维生素 D 通过抑制海马中的氧化应激和炎症，对 LPS 诱导的炎症引起的记忆功能障碍发挥保护作用。维生素 D 可能是一种有前途的潜在治疗补充剂，可用于治疗或预防学习和记忆障碍。
