Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, PR China; Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, China.
Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, PR China; Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Guangdong, 524000, China; Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, China.
Fish Shellfish Immunol. 2020 Jul;102:286-295. doi: 10.1016/j.fsi.2020.04.036. Epub 2020 Apr 22.
The current study was conducted to evaluate the effects of different levels of yeast culture (YC) supplementation at 0% (YC 0%), 1% (YC 1%), and 2% (YC 2%) on growth, feed conversion ratio, body composition, intestinal morphology, microflora, immune response, and resistance to Vibrio harveyi infection in Litopenaeus vannamei. After 8-weeks feeding trial, the results showed significant improvement (p < .05) in the final weight, weight gain rate, specific growth rate, survival rate and low feed conversion ratio in YC groups than the control. Serum total protein, superoxide dismutase, catalase, alkaline phosphatase, acid phosphatase, lysozyme, and phenol oxidase in shrimps fed diet YC (2%) were significantly higher (p < .05), whereas significantly decreased trend in serum cholesterol, triglyceride, aspartate aminotransferase, and alanine aminotransferase (p < .05) were observed in YC (2%) diet. Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes were the core phylum bacteria found in the shrimp intestines. At the genus level, opportunistic pathogenic bacteria, Vibrio was significantly decreased (p < .05) while beneficial bacteria Pseudoalteromonas was increased in YC (2%) group. Intestinal villus height and width in shrimps fed YC diets were significantly improved than the control diet (p < .05). YC groups challenged test significantly showed (p < .05) improved shrimps immune response against V. harveyi infections with YC (2%) recording the highest percentage survival rate (70%). The present study demonstrated that supplementing YC (2%) can improve growth, intestinal microbiota, intestinal morphology, and immune response against V. harveyi infections in L. vannamei.
本研究旨在评估不同水平的酵母培养物（YC）补充剂（0%（YC0%）、1%（YC1%）和 2%（YC2%））对凡纳滨对虾生长、饲料转化率、体组成、肠道形态、微生物群、免疫反应和对哈维弧菌感染的抗性的影响。经过 8 周的饲养试验，结果表明，YC 组的终重、增重率、特定生长率、成活率和低饲料转化率均显著提高（p<0.05）。YC 组（2%）虾血清总蛋白、超氧化物歧化酶、过氧化氢酶、碱性磷酸酶、酸性磷酸酶、溶菌酶和酚氧化酶显著升高（p<0.05），而血清胆固醇、甘油三酯、天冬氨酸转氨酶和丙氨酸转氨酶呈显著下降趋势（p<0.05）。虾肠道中的核心门细菌为变形菌门、拟杆菌门、放线菌门和厚壁菌门。在属水平上，机会致病菌弧菌显著减少（p<0.05），而有益菌假交替单胞菌在 YC（2%）组中增加。YC 组的虾肠道绒毛高度和宽度显著提高（p<0.05）。YC 组在哈维弧菌感染挑战试验中显著表现出（p<0.05）提高了虾的免疫反应，YC（2%）组的成活率最高（70%）。本研究表明，YC（2%）的添加可以提高凡纳滨对虾的生长、肠道微生物群、肠道形态和对哈维弧菌感染的免疫反应。
